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Quantification of Salmonella by 5′-Nuclease Real-Time Polymerase Chain Reaction Targeted to fimC Gene

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Abstract

A new 5′-nuclease real-time polymerase chain reaction (PCR) system for the quantification of Salmonella enterica was developed, with primers and the probe oriented to a Salmonella-specific region of the fimC gene. The PCR system was specific and sensitive, its inclusivity was 100% (determined by the analysis of 53 strains of Salmonella belonging to 38 serovars) and its exclusivity was 100% (determined by the analysis of 49 non-Salmonella strains). For quantification purposes, calibration lines were constructed for three Salmonella strains belonging to three serotypes. These calibration lines were linear (r ≥ 0.99) in the range from 103 to 107 CFU/mL and practically identical in terms of very similar slopes and x-intercepts. Escherichia coli (106 CFU/mL) and Citrobacter freundii (106 CFU/mL) had no effect on Salmonella quantification by the system.

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Acknowledgments

This research was financially supported by the Science and Technology Assistance Agency of the Slovak Republic, contract no. APVT-51-008902.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Biology, Food Research Institute, Priemyselná 4, P.O. Box 25, SK-82475 , Bratislava 26, Slovak Republic

    Ľubica Piknová, Eva Kaclíková & Tomáš Kuchta

  2. Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 , Bratislava, Slovak Republic

    Domenico Pangallo & Bystrík Polek

Authors
  1. Ľubica Piknová
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  2. Eva Kaclíková
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  3. Domenico Pangallo
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  4. Bystrík Polek
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  5. Tomáš Kuchta
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Corresponding author

Correspondence to Tomáš Kuchta.

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Piknová, Ľ., Kaclíková, E., Pangallo, D. et al. Quantification of Salmonella by 5′-Nuclease Real-Time Polymerase Chain Reaction Targeted to fimC Gene. Curr Microbiol 50, 38–42 (2005). https://doi.org/10.1007/s00284-004-4374-8

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  • DOI: https://doi.org/10.1007/s00284-004-4374-8

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