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MET3 Promoter: A Tightly Regulated Promoter and Its Application in Construction of Conditional Lethal Strain

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Abstract

The β-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Δcln2Δpho85Δ) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.

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Authors and Affiliations

  1. Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200031, China, , , , , , CN

    Xicheng Mao, Yafang Hu, Chao Liang & Changde Lu

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  1. Xicheng Mao
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  2. Yafang Hu
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  3. Chao Liang
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  4. Changde Lu
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Received: 20 September 2001 / Accepted: 22 October 2001

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Mao, X., Hu, Y., Liang, C. et al. MET3 Promoter: A Tightly Regulated Promoter and Its Application in Construction of Conditional Lethal Strain. Curr Microbiol 45, 37–40 (2002). https://doi.org/10.1007/s00284-001-0046-0

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  • DOI: https://doi.org/10.1007/s00284-001-0046-0

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