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Vascular activity of two silicon compounds, ALIS 409 and ALIS 421, novel multidrug-resistance reverting agents in cancer cells

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Abstract

Purpose

The aim of this study was to investigate the effects of two novel multidrug-resistance reverting agents, ALIS 409 [1,3-dimethyl-1,3-p-fluorophenyl-1,3(3-morfolinopropyl)-1,3-disiloxan dihydrochloride] and ALIS 421 {1,3-dimethyl-1,3-(4-fluorophenyl)-1,3[3(4-buthyl)-(1-piperazinyl)-propyl]-1,3-disiloxan tetrahydrochloride}, on vascular functions in vitro.

Experimental design

A comparison of their mechanical and electrophysiological actions in rat aorta rings and single rat tail artery myocytes, respectively, was performed.

Results

In endothelium-denuded rat aorta rings, ALIS 409 and ALIS 421 antagonized 60 mM K+-induced contraction in a concentration-dependent manner with IC50 values of 52.2 and 15.5 μM, respectively. ALIS 409 and ALIS 421 inhibited L-type Ca2+ current recorded in artery myocytes in a concentration-dependent manner with IC50 values of 6.4 and 5.6 μM, respectively. In rat aorta, ALIS 409 and ALIS 421 antagonized the sustained tonic contraction induced by phenylephrine with IC50 values of 58.0 and 13.7 μM (endothelium-denuded rings) and of 73.9 and 31.9 μM (endothelium-intact rings), respectively. In endothelium-denuded rings, ryanodine reduced significantly the response to phenylephrine in the absence of extracellular Ca2+ whereas nifedipine, ALIS 409 or ALIS 421 did not affect it. Phenylephrine-stimulated influx of extracellular Ca2+ was markedly reduced when tissues were pretreated with ALIS 409, ALIS 421 or nifedipine, and stimulated when they were pretreated with ryanodine. Application of ALIS 409 (up to 100 μM) to intact rat aorta rings failed to induce mechanical responses.

Conclusions

Our results provide functional evidence that the myorelaxing effect elicited either by ALIS 409 or by ALIS 421 involved mainly the direct blockade of extracellular Ca2+ influx. This effect, however, took place at concentrations much higher than those effective as modifiers of multidrug resistance in cancer cells.

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Abbreviations

I Ca(L) :

L-type Ca2+ current

K60:

60 mM K+ PSS

MDR:

Multidrug-resistance

PSS:

Physiological salt solution

ROCCs/SOCCs:

Receptor/store-operated Ca2+ channels

V h :

Holding potential

VOCCs:

Voltage-operated Ca2+ channels

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Acknowledgments

We wish to thank Drs Marco Gelli and Paolo D’Elia for the assistance in some preliminary experiments. This work was supported by grants from the Università degli Studi di Siena (PAR Servizi), a grant from Ministero degli Affari Esteri (Rome, Italy) as stipulated by Law 409/1990 and a grant from Szeged Foundation of Cancer Research, Szeged, Hungary.

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Authors and Affiliations

  1. Dipartimento di Scienze Biomediche, Università degli Studi di Siena, via A. Moro 2, 53100, Siena, Italy

    Fabio Fusi, Antonella Ferrara, Giampietro Sgaragli & Simona Saponara

  2. Faculty of Medicine, First Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

    Attila Zalatnai

  3. Faculty of Medicine, Institute of Microbiology, Albert Szent-Györgyi Medical University, Dom ter 10, 6720, Szeged, Hungary

    Joseph Molnar

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  1. Fabio Fusi
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  2. Antonella Ferrara
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  3. Attila Zalatnai
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  4. Joseph Molnar
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  5. Giampietro Sgaragli
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  6. Simona Saponara
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Correspondence to Fabio Fusi.

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Fusi, F., Ferrara, A., Zalatnai, A. et al. Vascular activity of two silicon compounds, ALIS 409 and ALIS 421, novel multidrug-resistance reverting agents in cancer cells. Cancer Chemother Pharmacol 61, 443–451 (2008). https://doi.org/10.1007/s00280-007-0488-6

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