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Wnt and Notch signaling pathways selectively regulating hematopoiesis

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Abstract

Hematopoietic stem and progenitor cells (HSPCs) are the source of all blood cells in the adult body. The pool of HSPCs is formed during embryogenesis process through a well-characterized succession of intra-embryonic regions and organs. The spatial and temporal restrictions in definitive hematopoietic development and the signaling molecules involved are of great interest as these may prove useful for generating and expanding these clinically important cell populations ex vivo. To elucidate the mechanism by which definitive HSPCs expand during this limited developmental time frame, we analyzed the spatial and temporal programmed gene expression patterns of Wnt and Notch signaling members during hematopoietic development. Genes related to the Wnt signaling pathway were up-regulated in E10.5 aorta-gonad-mesonephros (AGM) and E14.5 fetal liver corresponding to the inherent proliferation potential of hematopoietic progenitors, whereas genes related to the Notch signaling pathway were identified as up-regulated in E10.5 AGM, and bone marrow coincides with the maintenance of undifferentiation state of hematopoietic progenitors. Our findings suggest that Wnt and Notch signalings are integrated and are selectively regulating hematopoiesis. The spatial and temporal balance between Wnt and Notch signaling orchestrates the precise progression of hematopoietic progenitors.

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Acknowledgements

We sincerely thank Prof. Axel R. Zander and Prof. Boris Fehse at University Medical Center Hamburg-Eppendorf in Germany for critical review of the manuscript. This work was supported by National Natural Science Foundation (No.30570773) and Doctoral Fund of Ministry of Education of China (No.20060487061, No.200804870008).

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Authors and Affiliations

  1. Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jie Fang Avenue, Wuhan, Hubei, 430030, China

    Kun Zhou, Lifang Huang, Caihong Hu, Wenli Liu, Jianfeng Zhou & Hanying Sun

  2. Shanghai First People Hospital, Shanghai Jiao Tong University, Shanghai, 201620, China

    Kun Zhou & Zhigang Zhou

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  1. Kun Zhou
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  2. Lifang Huang
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Correspondence to Hanying Sun.

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Zhou, K., Huang, L., Zhou, Z. et al. Wnt and Notch signaling pathways selectively regulating hematopoiesis. Ann Hematol 89, 749–757 (2010). https://doi.org/10.1007/s00277-010-0923-3

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