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Prevalence and analysis of t(14;18) and t(11;14) chromosomal translocations in healthy Indian population

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Abstract

Hematopoietic malignancies like leukemia and lymphoma are characteristically associated with various chromosomal translocations. Follicular lymphoma (FL) and mantle cell lymphoma (MCL) are two subtypes of non-Hodgkin’s lymphoma which possess t(14;18) and t(11;14) translocations, respectively. The incidence of FL and MCL is higher in the western countries as compared to India. Interestingly, the associated translocations are also found in healthy individuals in western population, which is 50–80% for t(14;18), whereas t(11;14) occurs at a very low frequency. However, there are no studies to explore these translocations in healthy Indian population, which could explain the lower incidence of FL and MCL. We employed Southern hybridization following nested PCR to detect above translocations in healthy individuals from India. Our results suggest that this assay can detect one t(14;18) translocation event in up to 107 normal cells where as one t(11;14) in 108 normal cells. According to our results, 87 out of 253 individuals carry t(14;18) indicating 34% prevalence in the population. The presence of this translocation was also detectable at the transcript level. Although, no gender-based difference was observed, an age-dependent increase in the prevalence of translocation was found in adults. However, even after studying 210 people, we could not detect any t(11;14) translocation, indicating that it is uncommon in Indian population. These results suggest that lower incidence of FL and MCL in India could be attributed to lower prevalence of these translocations in healthy individuals.

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Acknowledgements

We thank Dr. Bibha Choudhary, Dr. M.S. Shahabuddin, Mr. Ghouse ul Azam, and members of the SCR laboratory for discussions and help. We would like to extend special thanks to all the volunteers who donated blood for this study. We also thank Chief Medical Officer, IISc and TTK blood bank, Bangalore for their help in blood collection.

Funding

This work was supported by grants from DBT, India (BT/PRS129/GBD/27/7/2006), Lady Tata Memorial Trust, UK (TLCS/0520), and IISc start up grant for SCR. We also thank Dr. Raghavan Varadarajan for financial assistance. MN is supported by JRF from CSIR, India.

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  1. Department of Biochemistry, Indian Institute of Science, Bangalore, 560 012, India

    Mridula Nambiar & Sathees C. Raghavan

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  1. Mridula Nambiar
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  2. Sathees C. Raghavan
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Correspondence to Sathees C. Raghavan.

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Supplementary Table 1

Age-wise distribution of t(14;18) translocation present in the healthy Indian population. All the healthy volunteers studied have been classified under four age groups. “Total” indicates the number of volunteers analyzed and “positive” indicates the number of individuals harboring the t(14;18) translocation in that category. The frequencies of positive samples are indicated in parenthesis. (PDF 100 kb)

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Nambiar, M., Raghavan, S.C. Prevalence and analysis of t(14;18) and t(11;14) chromosomal translocations in healthy Indian population. Ann Hematol 89, 35–43 (2010). https://doi.org/10.1007/s00277-009-0755-1

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