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Dup-697, a specific COX-2 inhibitor, suppresses growth and induces apoptosis on K562 leukemia cells by cell-cycle arrest and caspase-8 activation

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Abstract

This investigation was designed to assess the effect of DuP-697 on growth and apoptosis in a human chronic myeloid leukemia (CML) cell line (K562 cells) and primary CML cells from CML patient bone marrow. DuP-697 significantly suppressed K562 cells and primary CML cells growth and induced apoptosis in a concentration-dependent manner and the growth-inhibiting effect was independent on Philadelphia chromosome. The IC50 of DuP-697 at 36 h was 31.7 μM. It arrested G1-S phase transmit on cell cycle and its apoptosis activity was partially abrogated by pretreating K562 cells with Z-IETD-fmk, a specific inhibitor of caspase-8. This study suggested that Dup-697 suppresses growth and induces apoptosis on K562 leukemia cells by cell-cycle arrest and caspase-8 activation.

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Acknowledgement

This work was supported in part by a grant-in-aid from Chinese Medical Board. USA (no. 99-698).

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Authors and Affiliations

  1. Division of Hematology/Institute of Molecular Hematology, Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, 410011, Republic of China

    Hong-Ling Peng, Guang-Sen Zhang, Ji-Heng Liu, Fan-Jie Gong & Rui-Juan Li

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  1. Hong-Ling Peng
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  2. Guang-Sen Zhang
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  3. Ji-Heng Liu
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Correspondence to Guang-Sen Zhang.

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Peng, HL., Zhang, GS., Liu, JH. et al. Dup-697, a specific COX-2 inhibitor, suppresses growth and induces apoptosis on K562 leukemia cells by cell-cycle arrest and caspase-8 activation. Ann Hematol 87, 121–129 (2008). https://doi.org/10.1007/s00277-007-0385-4

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  • DOI: https://doi.org/10.1007/s00277-007-0385-4

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