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Distinct effects of different concentrations of sodium selenite on apoptosis, cell cycle, and gene expression profile in acute promyeloytic leukemia-derived NB4 cells

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Abstract

Selenium at a low concentration has a chemopreventive role against cancer, while at a high concentration, it exerts a direct antitumor effect. However, the mechanisms remain elusive. In this article, we discovered that Na2SeO3 at 20 μmol/l concentration could significantly inhibit the proliferation of NB4 cells, affect the cell cycle distribution of cell population, and induce cellular changes characteristic of apoptotic cells, while this same compound at 2 μmol/l concentration had no such effects. The mechanisms underlying these overt differences caused by treatment of different concentrations of selenium were further investigated. cDNA microarray analysis showed that after treatment by 20 μmol/l Na2SeO3, 34 genes were changed in expression, while treatment by 2 μmol/l Na2SeO3 resulted in the changes of 29 genes. Nine genes were regulated in both groups, among which three showed opposite changes caused by 2 and 20 μmol/l Na2SeO3. The majority of regulated genes did not coincide between the two experiment groups. In conclusion, 2 and 20 μmol/l Na2SeO3 could have different effects on NB4 cells, and some genes might be involved in the underlying mechanisms. Our findings could provide basis for further uncovering the molecular mechanisms of the chemopreventive and antitumor effects of selenium and, in turn, for probing the rationality of treating leukemia with selenium.

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Acknowledgements

This work was supported by grants from National Natural Sciences Foundation of China (no. 30370348), Doctoral Point Foundation of National Educational Committee (no. 20010023029), and Natural Sciences Foundation of Beijing (no. 7032034).

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Authors and Affiliations

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medicine, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100005, People's Republic of China

    Ting-Ming Cao, Fang-Yuan Hua, Cai-Min Xu, Bing-She Han, Hua Dong, Yang Yang & Hua-Zhen Pan

  2. Department of Hematology, Peking Union Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, People's Republic of China

    Lu Zuo, Xuan Wang & Zhi-Nan Zhang

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  1. Ting-Ming Cao
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  2. Fang-Yuan Hua
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Correspondence to Cai-Min Xu.

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Cao, TM., Hua, FY., Xu, CM. et al. Distinct effects of different concentrations of sodium selenite on apoptosis, cell cycle, and gene expression profile in acute promyeloytic leukemia-derived NB4 cells. Ann Hematol 85, 434–442 (2006). https://doi.org/10.1007/s00277-005-0046-4

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  • DOI: https://doi.org/10.1007/s00277-005-0046-4

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