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The morphological characteristics of corticostriatal and thalamostriatal neurons and their intrastriatal terminals in rats

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Abstract

Purpose

The glutamatergic projection from the cerebral cortex and the thalamus extensively innervates the neostriatal neurons. However, some conflicts in the published literatures about cortical and thalamic intrastriatal synaptic terminals still need to be resolved. The present study intends to further elucidate the morphological characteristics of these two types of the terminals and their neurons.

Methods

The corticostriatal and thalamostriatal terminals were immunolabeled for vesicular glutamate transporter type 1 (VGluT1) and 2 (VGluT2), respectively, and their neurons were retrograde labeled by biotinylated dextran amine 3,000 molecular weight (BDA3k) injection into the dorsolateral striatum of rats. The characteristics of the corticostriatal and thalamostriatal terminals were observed at the LM and EM levels, and the data were statistically analyzed with SPSS10.0 software.

Results

We observed that 63.53% of VGluT1+ terminals synapsed on dendritic spines, which was different from VGluT2+ terminals with the equal percentage of synapses on spines and dendrites (14.88 and 17.86%, respectively). Notably, VGluT1+ axospinous synaptic terminals were remarkably larger than VGluT2+ axospinous synaptic terminals. Terminal size-frequency distribution analysis showed that VGluT1+ terminals were within the size ranges of 0.4–0.5 and 0.8–0.9 μm, and VGluT2+ terminals were in the ranges of 0.4–0.5 and 0.6–0.7 μm. Perforated-postsynaptic densities (-PSDs) were more frequently found in VGluT1+ axospinous synaptic terminals than in VGluT2+ axospinous terminals. Furthermore, BDA3k-labeled corticostrital neurons were larger in perikaryal diameter than the thalamostriatal neurons, and they were also categorized as the two main populations based on their size-frequency distribution.

Conclusions

The morphological characteristics of corticostriatal and thalamostriatal terminals and neurons have implications for understanding the roles of synaptic plasticity in adaptive motor control by the basal ganglia, and they have facilitations for understanding the complexities of basal ganglia function.

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Acknowledgments

This study was supported by the grants from the National Science Foundations of China (No. 31070941, No. 20831006, No. 30770679) and the Major State Basic Research Development Program of China (973 Program) (No. 2010CB530004).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, No. 74, Zhongshan Road 2, Guangzhou, 510080, China

    Bingbing Liu, Lisi Ouyang, Shuhua Mu, Yaxi Zhu, Keyi Li, Mali Zhan, Zongwei Liu, Yu Jia & Wanlong Lei

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  1. Bingbing Liu
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  2. Lisi Ouyang
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  3. Shuhua Mu
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  7. Zongwei Liu
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  9. Wanlong Lei
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Correspondence to Wanlong Lei.

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Liu, B., Ouyang, L., Mu, S. et al. The morphological characteristics of corticostriatal and thalamostriatal neurons and their intrastriatal terminals in rats. Surg Radiol Anat 33, 807–817 (2011). https://doi.org/10.1007/s00276-011-0823-9

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  • DOI: https://doi.org/10.1007/s00276-011-0823-9

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