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Lack of Association between Microsatellite Instability and Benign Adrenal Tumors

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Abstract

Background

The adrenal gland may give rise to pheochromocytomas, which are catecholamine-producing tumors originating from the adrenal medulla, or to adrenocortical tumors, which derive from the adrenocortical cortex and may be secreting or not. The genetic mechanisms underlying the formation of these tumors include somatic mutations in susceptibility genes, especially in the familial forms, and allelic loss, especially in chromosome 1.

Aim

The aim of this study was to investigate a third genetic mechanism by evaluating microsatellite instability using the reference markers (Bat25, Bat26, D2S123, D5S346, D17S250) validated by the National Cancer Institute. Microsatellite loci were analyzed in 32 benign tumors, including 11 pheochromocytomas and 21 adrenocortical tumors, in patients with and without familial syndrome.

Results

The different alleles of microsatellite loci were reliably detected by DNA fragments analysis, whereas data obtained after melting-point analysis on the Lightcycler were inconsistent. No microsatellite instability was detected in any tumor. One patient with a unilateral pheochromocytoma showed a loss of heterozygosity for D17S250. A second patient with a MEN-2A syndrome and a two-sided pheochromocytoma exhibited a loss of heterozygosity for D2S123 in the right tumor only and a retention of heterozygosity for all markers in the left tumor.

Conclusions

These results suggest that microsatellite instability, evaluated by the five reference markers of the National Cancer Institute, is not a feature of benign adrenal tumors.

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Acknowledgments

We thank Dr Anne Schneider (Laboratoire de Biochimie, Hôpital de Haute Pierre, Strasbourg) for valuable discussions and for kindly providing DNA from MSI-positive colon carcinomas. We thank Olfert Landt from Tib-Molbiol, Berlin, Germany, for helpful discussions and recommendations concerning the Hyprobes/FRET technology. This work was supported by a grant from Ligue Contre le Cancer, Comité Départemental de Meurthe et Moselle.

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Authors and Affiliations

  1. Department of Biochemistry, CHU Nancy-Brabois, INSERM U724, allée du Morvan, 54511, Vandoeuvre les Nancy, France

    Fares Namour MD, PhD, Xiaohong Lu MD, Denise Tramoy & Jean-Louis Guéant MD, PhD

  2. Department of General and Endocrine Surgery, CHU Nancy-Brabois, 11 allée du Morvan, 54511, Vandoeuvre les Nancy, France

    Ahmet Ayav MD, PhD, Laurent Bresler MD, PhD & Laurent Brunaud MD, PhD

  3. Department of Endocrinology, CHU Nancy-Brabois, 11 allée du Morvan, 54511, Vandoeuvre les Nancy, France

    Marc Klein MD, PhD & Miahela Muresan MD

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  1. Fares Namour MD, PhD
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  2. Ahmet Ayav MD, PhD
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  3. Xiaohong Lu MD
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Correspondence to Fares Namour MD, PhD or Laurent Brunaud MD, PhD.

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Namour, F., Ayav, A., Lu, X. et al. Lack of Association between Microsatellite Instability and Benign Adrenal Tumors. World J. Surg. 30, 1240–1246 (2006). https://doi.org/10.1007/s00268-005-0471-5

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  • DOI: https://doi.org/10.1007/s00268-005-0471-5

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