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Intraperitoneal Administration of the Angiogenesis Inhibitor Thalidomide Does Not Impair Anastomotic Healing following Large Bowel Resection in a Rabbit Model

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Abstract

The effects of thalidomide after intraperitoneal instillation on the healing of colonic anastomoses are not known. A series of 40 New Zealand White rabbits underwent an end-to-end colonic anastomosis. The animals were randomized into four groups. Groups 1 (n = 10) and 2 (n = 10) were treated with dissolved thalidomide 200 mg/kg intraperitoneally, whereas groups 3 (n = 10) and 4 (n = 10) were treated only with the dissolver. Animals were sacrificed at day 3 (groups 1, 3) and day 7 (groups 2, 4). Anastomotic healing was tested by measuring the bursting pressure in vitro. Immunohistochemical staining of the anastomotic site was performed with polyclonal antibodies against CD31 and Mib-1, to determine a possible antiangiogenic or antiproliferative effect. Statistical analysis was performed using Spearman’s log rank correlation and paired t-test. On postoperative day 3 (p > 0.19) and postoperative day 7 (p > 0.73), there was no difference in bursting pressure in the treatment and the control groups. Angiogenesis scores were reduced at day 3 in the thalidomide group (p < 0.05), but did not differ between the groups at day 7. White blood cell counts were decreased in the treatment groups at day 3 (p < 0.01) and day 7, compared to control groups (p < 0.01). There was no difference in the expression of Mib-1 in either group at day 3 or day 7. The intraperitoneal administration of thalidomide does not interfere with the healing of colonic anastomosis. Although the angiogenesis score is diminished at day 3, this did not lead to a reduced bursting pressure at day 3 or day 7.

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Acknowledgments.

This work was funded by Grünenthal Research Department, Grünenthal AG, Aachen, Germany, Dr. K. Zwingenberger Clarify the role or position of Dr. Zwingenberger (what help is being acknowledged)?.

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Authors and Affiliations

  1. Department of General, Visceral, Thoracic and Vascular Surgery , Medical Faculty of the Humboldt University, Berlin, Charitè, Campus Mitte, Schumannstrasse 20/21, 10117, Berlin, Germany

    Julian W. Mall M.D., Wolfgang Schwenk M.D., Ph.D., Andreas W. Philipp M.D., Rolf Büttemeyer M.D. & Christian Pollmann M.D.

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  1. Julian W. Mall M.D.
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  2. Wolfgang Schwenk M.D., Ph.D.
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  3. Andreas W. Philipp M.D.
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Correspondence to Julian W. Mall M.D..

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Mall, J., Schwenk, W., Philipp, A. et al. Intraperitoneal Administration of the Angiogenesis Inhibitor Thalidomide Does Not Impair Anastomotic Healing following Large Bowel Resection in a Rabbit Model. World J. Surg. 27, 1119–1123 (2003). https://doi.org/10.1007/s00268-003-7009-5

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