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Density-Based High-Quality Fat: Characterization and Correlation with Different Body Fat Ratio

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  • Fat Injection
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Abstract

Background

Lipoaspirate can be divided into high-quality fat and low-quality fat using Coleman’s centrifugation by adding 0.935 g/ml marker float; the ratio obtained by different individuals is different.

Objectives

This study aimed to examine the HQF obtained from different individuals and establish the relationship between individual body data and HQF.

Methods

We used Coleman’s centrifugation method (1200 g, 3 min) with 0.935 g/ml density float to process lipoaspirate and collect HQF from different individuals for the analysis of fat characteristics and in vivo grafting.

Results

The HQF obtained from different individuals had similar stromal vascular fraction cell numbers and extracellular matrix content. In animal experiments at different time points (especially 12 weeks), the appearance, retention rate, hematoxylin and eosin staining, and immunohistochemistry results of HQF grafts were similar, while being different from those of Coleman fat. The HQF obtained from individuals with higher body fat ratio was less than those with lower body fat ratio. Following the establishment of the relationship between high-quality fat percentage and the body fat ratio of the donors, we proposed an innovative calculation formula model for the required lipoaspirate.

Conclusions

HQF obtained from different individuals has similar fat characteristics, transplantation process, and outcome. The HQF percentage obtained from different individuals is negatively correlated with the body fat ratio. The amount of liposuction can be predicted using the proposed formula and improve the predictability of fat transplantation.

Level of Evidence IV

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References

  1. Fraser J, Wulur I, Alfonso Z, Hedrick M (2006) Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol 24:150–154

    Article  CAS  Google Scholar 

  2. Strem B, Hicok K, Zhu M, Wulur I, Alfonso Z, Schreiber R, Fraser J, Hedrick M (2005) Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med 54:132–141

    Article  CAS  Google Scholar 

  3. Rigotti G, Marchi A, Sbarbati A (2009) Adipose-derived mesenchymal stem cells: past, present, and future. Aesthet Plast Surg 33:271–273

    Article  Google Scholar 

  4. Simonacci F, Bertozzi N, Grieco M, Grignaffini E, Raposio E (2017) Procedure, applications, and outcomes of autologous fat grafting. Ann Med Surg (Lond) 20:49–60

    Article  Google Scholar 

  5. Yoshimura K, Eto H, Kato H, Doi K, Aoi N (2011) In vivo manipulation of stem cells for adipose tissue repair/reconstruction. Regen Med 6:33–41

    Article  CAS  Google Scholar 

  6. Fisher C, Grahovac TL, Schafer ME, Shippert RD, Marra KG, Rubin JP (2013) Comparison of harvest and processing techniques for fat grafting and adipose stem cell isolation. Plast Reconstr Surg 132:351–361

    Article  CAS  Google Scholar 

  7. Pu LLQ, Coleman SR, Cui X, Ferguson REH Jr, Vasconez HC (2008) Autologous fat grafts harvested and refined by the Coleman technique: a comparative study. Plast Reconstr Surg 122:932–937

    Article  CAS  Google Scholar 

  8. De Francesco F, Guastafierro A, Nicoletti G, Razzano S, Riccio M, Ferraro GA (2017) The selective centrifugation ensures a better in vitro isolation of ascs and restores a soft tissue regeneration in vivo. Int J Mol Sci 18:1038

    Article  Google Scholar 

  9. Qiu L, Su Y, Zhang D, Song Y, Liu B, Yu Z, Guo S, Yi C (2016) Identification of the centrifuged lipoaspirate fractions suitable for postgrafting survival. Plast Reconstr Surg 137:67e–76e

    Article  CAS  Google Scholar 

  10. Ibatici A, Caviggioli F, Valeriano V, Quirici N, Sessarego N, Lisa A, Klinger F, Forcellini D, Maione L, Klinger M (2014) Comparison of cell number, viability, phenotypic profile, clonogenic, and proliferative potential of adipose-derived stem cell populations between centrifuged and noncentrifuged fat. Aesthet Plast Surg 38:985–993

    Article  Google Scholar 

  11. Galiè M, Pignatti M, Scambi I, Sbarbati A, Rigotti G (2008) Comparison of different centrifugation protocols for the best yield of adipose-derived stromal cells from lipoaspirates. Plast Reconstr Surg 122:233e–234e

    Article  Google Scholar 

  12. Kurita M, Matsumoto D, Shigeura T, Sato K, Gonda K, Harii K, Yoshimura K (2008) Influences of centrifugation on cells and tissues in liposuction aspirates: optimized centrifugation for lipotransfer and cell isolation. Plast Reconstr Surg 121:1033–1041

    Article  CAS  Google Scholar 

  13. Allen RJ Jr, Canizares O Jr, Scharf C, Nguyen PD, Thanik V, Saadeh PB, Coleman SR, Hazen A (2013) Grading lipoaspirate: is there an optimal density for fat grafting? Plast Reconstr Surg 131:38–45

    Article  CAS  Google Scholar 

  14. Clauser L, Ferroni L, Gardin C, Tieghi R, Galiè M, Elia G, Piattelli A, Pinton P, Bressan E, Zavan B (2014) Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery. PLoS ONE 9:e110796

    Article  Google Scholar 

  15. Guan J, He Y, Wang X, Yao Y, Li Y, Wang Z, Chen Y, Dong Z, Lu F (2020) Identification of high-quality fat based on precision centrifugation in lipoaspirates using marker floats. Plast Reconstr Surg 146:541–550

    Article  CAS  Google Scholar 

  16. van Harmelen V, Skurk T, Röhrig K, Lee YM, Halbleib M, Aprath-Husmann I, Hauner H (2003) Effect of BMI and age on adipose tissue cellularity and differentiation capacity in women. Int J Obes Relat Metab Disord J Int Assoc Study Obes 27:889–895

    Article  Google Scholar 

  17. Woolcott OO, Bergman RN (2018) Relative fat mass (RFM) as a new estimator of whole-body fat percentage - a cross-sectional study in American adult individuals. Sci Rep 8:10980

    Article  Google Scholar 

  18. Panina YA, Yakimov AS, Komleva YK, Morgun AV, Lopatina OL, Malinovskaya NA, Shuvaev AN, Salmin VV, Taranushenko TE, Salmina AB (2018) Plasticity of adipose tissue-derived stem cells and regulation of angiogenesis. Front Physiol 9:1656

    Article  Google Scholar 

  19. Bi H, Li H, Zhang C, Mao Y, Nie F, Xing Y, Sha W, Wang X, Irwin D, Tan H (2019) Stromal vascular fraction promotes migration of fibroblasts and angiogenesis through regulation of extracellular matrix in the skin wound healing process. Stem Cell Res Ther 10:302

    Article  Google Scholar 

  20. Dulmovits B, Herman I (2012) Microvascular remodeling and wound healing: a role for pericytes. Int J Biochem Cell Biol 44:1800–1812

    Article  CAS  Google Scholar 

  21. Genova T, Grolez G, Camillo C, Bernardini M, Bokhobza A, Richard E, Scianna M, Lemonnier L, Valdembri D, Munaron L, Philips M, Mattot V, Serini G, Prevarskaya N, Gkika D, Pla A (2017) TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. J Cell Biol 216:2107–2130

    Article  CAS  Google Scholar 

  22. Moniri M, Boroumand Moghaddam A, Azizi S, Abdul Rahim R, Zuhainis Saad W, Navaderi M, Arulselvan P, Mohamad R (2018) Molecular study of wound healing after using biosynthesized BNC/FeO nanocomposites assisted with a bioinformatics approach. Int J Nanomed 13:2955–2971

    Article  CAS  Google Scholar 

  23. Masli S, Sheibani N, Cursiefen C, Zieske J (2014) Matricellular protein thrombospondins: influence on ocular angiogenesis, wound healing and immuneregulation. Curr Eye Res 39:759–774

    Article  CAS  Google Scholar 

  24. Ishihara J, Ishihara A, Fukunaga K, Sasaki K, White M, Briquez P, Hubbell J (2018) Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing. Nat Commun 9:2163

    Article  Google Scholar 

  25. Srdić B, Stokić E, Korać A, Ukropina M, Veličković K, Breberina M (2010) Morphological characteristics of abdominal adipose tissue in normal-weight and obese women of different metabolic profiles. Exp Clin Endocrinol Diabetes 118:713–718

    Article  Google Scholar 

  26. Kim N, Ahn J, Choi Y, Son H, Choi W, Cho H, Yu J, Seo J, Jang Y, Jung C, Ha T (2020) Differential circulating and visceral fat microRNA expression of non-obese and obese subjects. Clin Nutr 39:910–916

    Article  CAS  Google Scholar 

  27. Greenberg AS, Obin MS (2006) Obesity and the role of adipose tissue in inflammation and metabolism. Am J Clin Nutr 83:461s–465s

    Article  CAS  Google Scholar 

  28. Geissler PJ, Davis K, Roostaeian J, Unger J, Huang J, Rohrich RJ (2014) Improving fat transfer viability: the role of aging, body mass index, and harvest site. Plast Reconstr Surg 134:227–232

    Article  CAS  Google Scholar 

  29. Collins K, Sharif B, Sanmartin C, Reimer R, Herzog W, Chin R, Marshall D (2017) Association of body mass index (BMI) and percent body fat among BMI-defined non-obese middle-aged individuals: insights from a population-based Canadian sample. Can J Public Health 107:e520–e525

    Article  Google Scholar 

  30. Deurenberg P, Weststrate JA, Seidell JC (1991) Body mass index as a measure of body fatness: age- and sex-specific prediction formulas. Br J Nutr 65:105–114

    Article  CAS  Google Scholar 

  31. Ullmann Y, Shoshani O, Fodor A, Ramon Y, Carmi N, Eldor L, Gilhar A (2005) Searching for the favorable donor site for fat injection: in vivo study using the nude mice model. Dermatol Surg 31:1304–1307

    Article  CAS  Google Scholar 

  32. Li K, Gao J, Zhang Z, Li J, Cha P, Liao Y, Wang G, Lu F (2013) Selection of donor site for fat grafting and cell isolation. Aesthet Plast Surg 37:153–158

    Article  Google Scholar 

  33. Rohrich R, Sorokin E, Brown S (2004) In search of improved fat transfer viability: a quantitative analysis of the role of centrifugation and harvest site. Plast Reconstr Surg 113:391–395

    Article  Google Scholar 

  34. Lee S, Gallagher D (2008) Assessment methods in human body composition. Curr Opin Clin Nutr Metab Care 11:566–572

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Nature Science Foundation of China (81801933, 81801932, 81871573, 81901976, 81901975, 81971852). Postdoctoral Science Foundation of China (2020M672723). Medical Scientific Research Foundation of Guangdong Province of China (A2020542). Science and Technology Program of Guangzhou of China (201604020007). Administrator Foundation of Nanfang Hospital (2019B021, 2020Z004). National undergraduate innovation and entrepreneurship training program (X202012121222, X202012121312).

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Author notes

  1. Ziqing Dong and Feng Lu contribute equally and should be co-corresponding Author.

Authors and Affiliations

  1. Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, 510515, Guangdong, China

    Xinhui Wang, Jingyan Guan, Yunzi Chen, Ye Li, Feng Lu & Ziqing Dong

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  1. Xinhui Wang
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  2. Jingyan Guan
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Correspondence to Feng Lu or Ziqing Dong.

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The authors declare that they have no conflicts of interest to disclose.

Ethical Approval

All animal experiments were approved by the Institutional Animal Care and Use Committee of Nanfang Hospital and were conducted in accordance with the guidelines of the National Health and Medical Research Committee (People's Republic of China). And all applicable institutional and national guidelines for the care and use of animals were followed.

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The informed consent of patients was obtained before the operation in this study.

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Wang, X., Guan, J., Chen, Y. et al. Density-Based High-Quality Fat: Characterization and Correlation with Different Body Fat Ratio. Aesth Plast Surg 46, 3003–3012 (2022). https://doi.org/10.1007/s00266-022-02973-w

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  • DOI: https://doi.org/10.1007/s00266-022-02973-w

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