Skip to main content

Advertisement

SpringerLink
Log in

Effects of Copaifera langsdorffii Desf. on Ischemia-Reperfusion of Randomized Skin Flaps in Rats

  • Original Article
  • Published:
Aesthetic Plastic Surgery Aims and scope Submit manuscript

Abstract

Background

Copaíba oil is an oleoresin obtained from the Copaiffera langsdorffii genus (Leguminoseae). It is widely used in folk medicine as an antiinflammatory, healing, and antiseptic agent. Comparative pharmacologic studies between different species of copaíba oils are scarce.

Methods

The protective effect of Copaiffera langsdorffii was evaluated on an experimental model of random skin flaps on rat dorsums.

Results

Seventy-two Wistar rats (average weight = 216.8 g) were divided randomly into four equal groups (saline control, vehicle control, GT200—Test 1, and GT400—Test 2). A caudally based rectangular flap, 2.5–8.0 cm in size, was elevated on the back of the rat using McFarlane’s method. The flap was sutured back into its original place. Copaifera and control drugs (saline and Tween 80) were administered by gavage 24, 12, and 2 h prior to the beginning of the experiment followed by daily doses for the next 7 days. To observe the effects of Copaifera, laboratory analyses included plasma and tissue levels of tiobarbituric acid-reactive substances (TBARS) and reduced glutathione (GSH) and tissue levels of myeloperoxidase (MPO).

Conclusion

The oil-resin of copaíba presents discrete antilipoperoxidation action, intense antioxidant action, and antiinflammatory activity during the ischemia and reperfusion of randomized cutaneous flaps. The effects of ischemia-reperfusion are complex and substances capable of increasing the tolerance of tissue to those effects by reducing the production or neutralizing the action of free radicals are needed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Almeida KG, Fagundes DJ, Manna MC, Souza EF (2004) Ação do dimetil-sulfóxido na isquemia de retalhos randômicos de pele em ratos. Acta Cir Bras 19(6):649–657

    Article  Google Scholar 

  2. Biondo-Simões ML, Greca FH, Paula JB, Chin EW, Santos EA, Stalhschmidt FL (2000) Effect of buflomedil on ischemic skin flaps: experimental study in rats. Acta Cir Bras 15(Suppl 3):36–40

    Google Scholar 

  3. Francisco Neto A, Silva JC, Fagundes DJ, Percário S, Novo NF, Juliano Y, Moreira Neto AA (2005) Oxidative alterations, total antioxidant status and nitric oxide study in rats submitted to ischemia and reperfusion of hind limbs. Acta Cir Bras 20(2):134–139 [in Portugeuse]

    Article  PubMed  Google Scholar 

  4. Hart K, Baur D, Hodam J, Lesoon-Wood L, Parham M, Keith K, Vasquez R, Ager E, Pizarro J (2006) Short- and long-term effects of sildenafil on skin flap survival in rats. Laryngoscope 116:522–528

    Article  PubMed  CAS  Google Scholar 

  5. Huemer GM, Wechselberger G, Otto-Schoeller A, Gurunluoglu R, Piza-Katzer H, Schoeller T (2003) Improved dorsal random-pattern skin flap survival in rats with a topically applied combination of Nonivamide and Nicoboxil. Plast Reconstr Surg 111(3):1207–1211

    Article  PubMed  Google Scholar 

  6. Bittencourt RC, Biondo-Simões ML, Paula JB, Martynetz J, Groth A (2005) Influence of minoxidil on ischemic cutaneous flaps in rats. Acta Cir Bras 20(6):450–454

    Article  Google Scholar 

  7. Komorowska-Timek E, Timek TA, Brevetti LS, Szuba A, Stuelinger M, Hardesty RA, Buncke HJ (2004) Oral administration of L-arginine decreases necrosis of the epigastric skin flap in the rat. Ann Plast Surg 53(1):73–78

    Article  PubMed  Google Scholar 

  8. Souza Filho MV, Leal PR, Sbalchiero JC, Marques MJ (2002) Effect of ginkgo biloba extract on skin flap survival in rats. Rev Soc Bras Cir Plast 17(2):45–56

    Google Scholar 

  9. Ghavami A, Nutt MP, Hardy SP (2002) Heat shock protein and high-dose aspirin: effects on random skin flap survival in a rat model. Ann Plast Surg 48(1):60–67

    Article  PubMed  Google Scholar 

  10. McFarlane RM, DeYoung G, Henry RA (1965) The design of a pedicle flap in the rat to study necrosis and its prevention. Plast Reconstr Surg 35:177–178

    Article  PubMed  CAS  Google Scholar 

  11. Tyner TR, Shahbazian R, Nakashima J, Kane S, Sian K, Yamaguchi KT (2004) Propofol improves skin flap survival in a rat model: correlating reduction in flap-induced neutrophil activity. Ann Plast Surg 53(3):273–277

    Article  PubMed  Google Scholar 

  12. Sarifakioglu N, Gokrem S, Ates L, Akbuga UB, Aslan G (2004) The influence of sildenafil on random skin flap survival in rats: an experimental study. Br J Plast Surg 57:769–772

    Article  PubMed  Google Scholar 

  13. Esteves Junior I, Ferreira LM, Liebano RE (2004) Calcitonin gene-related peptide by iontophoresis on the viability of the randon skin flaps in rats. Acta Cir Bras 19(6):626–629

    Article  Google Scholar 

  14. Fonseca AV, Ferreira LM, Gomes HC, Percario S (2004) Terazosin and propanolol as blockers to the deleterious effect of nicotine in a random skin flap in the rat. Acta Cir Bras 19(3):186–195

    Article  Google Scholar 

  15. Yoshida WB, Campos EB (2005) Ischemia and reperfusion in skin flaps: effects of mannitol and vitamin C in reducing necrosis area in a rat experimental model. Acta Cir Bras 20(5):358–363

    Article  PubMed  Google Scholar 

  16. Junior IE, Masson IB, Ferreira LM, Liebano RE, Baldan C, Gomes AC (2005) Topical administration of hydralazine hydrochloride on the viability of randon skin flaps in rats. Acta Cir Bras 20(2):164–167 [in Portugeuse]

    PubMed  Google Scholar 

  17. Aydogan H, Gurlek A, Parlakpinar H, Askar I, Bay-Karabulut A, Aydogan N, Fariz A, Acet A (2007) Beneficial effects of caffeic acid phenethyl ester (CAPE) on the ischaemia-reperfusion injury in rat skin flaps. J Plast Reconstr Aesthet Surg 60(5):563–568

    Article  PubMed  Google Scholar 

  18. Ersoy A, Sevin K, Sevin A, Serel S (2007) Effects of clopidogrel on survival of rat skin flaps. J Plast Reconstr Aesthet Surg 60(8):861–863

    Article  PubMed  Google Scholar 

  19. Paiva LA, Gurgel LA, Silva RM, Tomé AR, Gramosa NV, Silveira ER, Santos FA, Rao VS (2002) Anti-inflammatory effect of kaurenoic acid, a diterpene from Copaifera langsdorffii on acetic acid-induced colitis in rats. Vascul Pharmacol 39(6):303–307

    Article  PubMed  CAS  Google Scholar 

  20. Oshaki A, Yan LT, Ito S, Edatsugi H, Iwata D, Komoda Y (1994) The isolation and in vivo potent antitumor activity of clerodane diterpenoids from the oleoresin of Brazilian medicinal plant Copaifera langsdorffii Desfon. Bioorg Med Chem Lett 4:2889–2892

    Article  Google Scholar 

  21. Lima ES, Abdalla DSP (2001) Lipídic peroxidation: evaluation mechanisms in biological samples. Rev Bras Ci Farm 37(3):293–303

    CAS  Google Scholar 

  22. Paiva LA, Rao VS, Gramosa NV, Silveira ER (1998) Gastroprotective effect of Copaifera langsdorffii oleo-resin on experimental gastric ulcer models in rats. J Ethnopharmacol 62(1):73–78

    Article  PubMed  CAS  Google Scholar 

  23. Paiva LA, Gurgel LA, De Sousa ET, Silveira ER, Silva RM, Santos FA, Rao VS (2004) Protective effect of Copaifera langsdorffii oleo-resin against acetic acid-induced colitis in rats. J Ethnopharmacol 93(1):51–56

    Article  PubMed  CAS  Google Scholar 

  24. Cymrot M, Percário S, Ferreira LM (2004) Oxidative stress and total antioxidant status in ischemic skin flaps in rats. Acta Cir Bras 19(1):18–26

    Article  Google Scholar 

  25. Rover Júnior L, Höehr NF, Vellasco AP (2001) Anti-oxidant system involving the metabolic cycle of glutathione associated with electroanalytical methods in the assessment of oxidative stress. Quim Nova 24(1):112–119

    Google Scholar 

  26. Franck T, Kohnen S, Grulke S, Neven P, Goutman Y, Peters F, Pirotte B, Deby-Dupont G, Serteyn D (2007) Inhibitory effect of curcuminoids and tetrahydrocorcuminoids on equine activated neutrophils and myeloperoxidase activity. Physiol Res 57(4):577–587

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Universidade Federal do Ceará, Alexandre Baraúna Street 949, Rodolfo Teófilo, Fortaleza, Brazil

    Joaquim José de Lima Silva, Sérgio Botelho Guimarães, Edilberto Rocha da Silveira, Paulo Roberto Leitao de Vasconcelos, Gabrielle Gurgel Lima, Sílvio Melo Torres & Raquel Cavalcante de Vasconcelos

Authors
  1. Joaquim José de Lima Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sérgio Botelho Guimarães
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edilberto Rocha da Silveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paulo Roberto Leitao de Vasconcelos
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gabrielle Gurgel Lima
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sílvio Melo Torres
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Raquel Cavalcante de Vasconcelos
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joaquim José de Lima Silva.

Rights and permissions

Reprints and permissions

About this article

Cite this article

de Lima Silva, J.J., Guimarães, S.B., da Silveira, E.R. et al. Effects of Copaifera langsdorffii Desf. on Ischemia-Reperfusion of Randomized Skin Flaps in Rats. Aesth Plast Surg 33, 104–109 (2009). https://doi.org/10.1007/s00266-008-9263-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00266-008-9263-2

Keywords

Search

Navigation