Abstract
Stem cells, because of their pluripotentiality and unlimited capacity for self-renewal, project great promise for tissue engineering and are expected to allow significant advances for distinct reconstructive procedures. This study aimed to establish a versatile experimental model with potential application to distinct lines of research in plastic surgery. The search was guided by the need for mesenchymal stem cells that can be easily obtained for laboratory research. The authors studied the anatomy of a number of animals as potential donors and indicated the white New Zealand rabbit (Oryctolagus cuniculus) for this purpose. The animals of this species present definite areas of lipid tissue on their back, allowing easy access to the tissue by either exeresis or liposuction.
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Amint M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA,Itskovitz-Eldor J, et al: Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227:271–278, 2000
Boquest AC, Noer A, Sorensen AL, Vekterud K, Collas P: CpG methylation profiles of endothelial cell-specific gene promoter regions in adipose tissue stem cells suggest limited differentiation potential toward the endothelial cell lineage. Stem Cells 25(4):852–861, 2007
Chajchir A, Fabrizio D, Chajchir G, Celi E: Growth factors in plastic surgery. Aesth Plast Surg 29:295–299, 2005
Choi YS, Cha SM, Lee YY, Kwon SW, Park CJ, Kim M: Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse. D Biochem Biophys Res Commun 345:631–637, 2006
Clavijo-Alvarez JA, Rubin JP, Bennett JMS, Nguyen VT. Dudas JBS, Underwood C, Marra KG: A novel perfluoroelastomer seeded with adipose-derived stem cells for soft tissue repair. Plast Reconstr Surg 118:1132–1142, 2006
Conejero JA, Lee JA, Parrett BM, Terry M, Wear-Maggitti K, Grant RT, Breitbart AS: Repair of palatal bone defects using osteogenically differentiated fat-derived stem cells. Plast Reconstr Surg 117:857–863, 2006
Dai W, Hale SL, Martin BJ, Kuang JQ, Dow JS, Wold LE, et al: Allogenic mesenchymal stem cell transplantation in postinfarcted rat myocardium: Short- and long-term effects. Circulation 112:214–223, 2005
Findikli N, Candan NZ, Kahraman S: Related articles: Human embryonic stem cell culture: Current limitations and novel strategies [online]. Reprod Biomed 13:581–590, 2006
Gabbay JS, Heller JB, Mitchell SA, Zuk PA, Spoon DB, Wasson KL, Jarrahy R, Benhaim P, Bradley JP: Osteogenic potentiation of human adipose-derived stem cells in a 3-dimensional matrix. Ann Plast Surg 57:89–93, 2006
Giraud S, Bessette B, Boda C, Lalloue F, Petit D, Mathonnet M, Jauberteau MO: In vitro apoptotic induction of human glioblastoma cells by Fas ligand plus etoposide and in vivo antitumour activity of combined drugs in xenografted nude rats. Int J Oncol 30:273–281, 2007
Goessler UR, Hormann K, Riedel F: Tissue engineering with adult stem cells in reconstructive surgery (review). Int J Mol Med 15:899–905, 2005
Koch CA, Jordan CE, Platt JL: Complement-dependent control of teratoma formation by embryonic stem cells. J Immunol 177:4803–4809, 2006
Kokai LE, Rubin JP, Marra KG: The potential of adipose-derived adult stem cells as a source of neuronal progenitor cells. Plast Reconstr Surg 116:1453–1460, 2005
Lee JA, Parrett BM, Conejero JA, Laser J, Chen J, Kogon AJ, Nanda D, Grant RT, Breitbart AS: Biological alchemy: Engineering bone and fat from fat-derived stem cells. Ann Plast Surg 50:610–617, 2003
Morain WD: The stem of our future. Ann Plast Surg 54:577–578, 2005
Planat-Bernard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, Tamarat R, et al: Plasticity of human adipose lineage cells toward endothelial cells: Physiological and therapeutic perspectives. Circulation 109:656–663, 2004
Portinho CP, Collares MVM, Silvia FH, Nardi MB, Pinto RA, Siqueira E, Morellato G, Sumino K: Reconstrução de calota craniana com células-tronco mesenquimais indiferenciadas: Estudo experimental. Rev Soc Bras Cir Plást 21:161–165, 2006
Rickardd DJ, Kassem M, Hefferan TE, Sarkar G, Spelsberg TC, Riggs B: Isolation and characterization of osteoblast precursor cells from human bone marrow. J Bone Miner Res 11:312–324, 1996
Rosenthal N: Prometheu’s vulture and stem cell promise. N Engl J Med 17 349:267–274, 2003
Shi YY, Nacamuli RP, Salim A, Longaker MT: The osteogenic potential of adipose-derived mesenchymal cells is maintained with aging. Plast Reconstr Surg 116:1686–1696, 2005
Vacanti JP, Vacanti CA: The history and scope of tissue engineering. In: Lanza PR, Langer R, Vacanti J (eds) Principles of tissue engineering. 2nd ed. Academic Press, San Diego, p. 37, 2000
Walgenbach KJ, Voigt M, Riabikhin AW, Andree C, Schaefer DJ, Galla TJ, Bjorn G: Tissue engineering in plastic reconstructive surgery. Anat Rec 263:372–378, 2001
Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, et al: Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng 7:211–228, 2001
Acknowledgments
The authors thank Alexandre Queiroz Silva, biologist, for his skillful technical assistance, to Professor Lilian Piñero Eça for her valuable suggestions, and to Guilherme Flosi Stocchero, fourth-year student at the University of São Paulo Medical School, for his kind availability in helping with the preliminarily translation of this article.
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Torres, F.C., Rodrigues, C.J., Stocchero, I.N. et al. Stem Cells from the Fat Tissue of Rabbits: An Easy-To-Find Experimental Source. Aesth Plast Surg 31, 574–578 (2007). https://doi.org/10.1007/s00266-007-0001-y
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DOI: https://doi.org/10.1007/s00266-007-0001-y