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The Fate of Intramuscularly Injected Fat Autografts: An Experimental Study in Rabbits

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Abstract

An experimental study was designed to assess the viability and revascularization of intramuscularly injected fat autografts. For the study, 18 rabbits were divided into two groups. In the first group, fat was injected intramuscularly (12 rabbits). Autologous fat was obtained from the inguinal area and subsequently injected into the thigh muscle. In the second group, physiologic saline was injected intramuscularly to determine the effects of cannulation and pressure on muscle tissue (6 rabbits). Fat autografts were performed on the right side of the animal, and the left side was used as the control. Scintigraphic imaging and histopathologic examination of the limbs were performed after injection of adipose tissue on days 15, 30, 45, 60, 90, and 120. On the technetium-99m (99mTc) hexamethylpropylene amine oxime scintigraphy, whereas similar activity distribution was observed between the left and right thigh on days 15, 30, and 45, there was increased uptake at the right thigh on days 60, 90, and 120. This increased uptake indicates that there is viable fat tissue in this region. Histopathologic evaluation showed that microcysts resulting from degeneration of some adipocytes and inflammatory changes on day 15 additionally increased vascularity and fibrosis in some animals on day 30, as well as fibrosis, microcysties, and focal calcification areas in adipose tissue on day 45 and later. It was observed that adipose tissue survived in more than 50% of the graft area in all the animals. These findings show that fat autografts can survive in muscle tissue with less than 50% fibrotic change.

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References

  1. M Ayhan D Şenen G Adanali M Görgü B Erdoğan B Albayrak (2001) ArticleTitleUse of beta-blockers for increasing survival of free fat grafts Aesth Plast Surg 25 338–342

    Google Scholar 

  2. CA Carpaneda MT Riberio (1993) ArticleTitleStudy of the histologic alterations and viability of the adipose graft in humans Aesth Plast Surg 17 43–47

    Google Scholar 

  3. CA Carpaneda MT Riberio (1994) ArticleTitlePercentage of graft viability versus injected volume in adipose autotransplants Aesth Plast Surg 18 17–19

    Google Scholar 

  4. A Chajchir I Benzaquen E Wexler H Aristides-Arellano (1990) ArticleTitleFat injection Aesth Plast Surg 14 127–136 Occurrence Handle1:STN:280:By%2BB2c%2Fht10%3D

    CAS  Google Scholar 

  5. A Chajchir I Benzaquen (1989) ArticleTitleFat-grafting injection for soft tissue augmentation Plast Reconstr Surg 84 921–934

    Google Scholar 

  6. SR Coleman (2001) ArticleTitleStructural fat grafts Clin Plast Surg 28 111–119 Occurrence Handle1:STN:280:DC%2BD3M3jtl2jtA%3D%3D Occurrence Handle11248861

    CAS  PubMed  Google Scholar 

  7. MM Colic (1999) ArticleTitleLip and perioral enhancement by direct intramuscular fat autografting Aesth Plast Surg 23 36–40

    Google Scholar 

  8. SS Ergün DI Çek H Baloğlu Z Algün H Önay (2001) ArticleTitleWhy is augmentation with autologous fat injection less effective in the vermillion border? Aesth Plast Surg 25 350–352

    Google Scholar 

  9. RA Ersek P Chang BS Salisbury (1998) ArticleTitleLipo layering of autologus fat: an improved technique with promising results Plast Reconstr Surg. 101 820–826

    Google Scholar 

  10. D Fagrell S Enestrom A Berggren (1996) ArticleTitleFat cylinder transplantation: an experimental comparative study of three different kinds of fat transplants Plast Reconstr Surg 98 90–96

    Google Scholar 

  11. JE Gatti (1999) ArticleTitlePermanent lip augmentation with serial fat grafting Ann Plast Surg 42 376–380

    Google Scholar 

  12. J Guerrerosantos (1996) ArticleTitleAutologous fat grafting for body contouring Clin Plast Surg 23 619–631

    Google Scholar 

  13. J Guerrerosantos (2000) ArticleTitleLong-term outcome of autologous fat transplantation in aesthetic facial recountouring: sixteen years of experience with 1936 cases Clin Plast Surg 27 515–543 Occurrence Handle1:STN:280:DC%2BD3cvovV2gug%3D%3D Occurrence Handle11039887

    CAS  PubMed  Google Scholar 

  14. J Guerrerosantos A Gonzalez-Mendoza Y Masmela MA Gonzalez M Deos P Diaz (1996) ArticleTitleLong-term survival of free fat grafts in muscle: an experimental study in rats Aesth Plast Surg 20 403–408 Occurrence Handle10.1007/s002669900055 Occurrence Handle1:STN:280:ByiD3c3ktFY%3D

    Article  CAS  Google Scholar 

  15. Y Har-Shai E Lindenbaum O Ben-Itzhak B Hirshowitz (1996) ArticleTitleLarge liponecrotic pseudocyst formation following cheek augmentation by fat injection Aesth Plast Surg 20 417–419

    Google Scholar 

  16. Y-G Illouz (1988) ArticleTitlePresent result of fat injection Aesth Plast Surg 12 175–181

    Google Scholar 

  17. TC Kononas LP Bucky C Hurley JW May (1993) ArticleTitleThe fate of suctioned and surgically removed fat After reimplantation for soft tissue augmentation: a volumetric and histologic study in the rabbit Plast Reconstr Surg 91 763–768 Occurrence Handle1:STN:280:ByyB3M3gtFE%3D Occurrence Handle8460177

    CAS  PubMed  Google Scholar 

  18. S Langer I Sinitsina P Biberthaler F Krombach K Messmer (2002) ArticleTitleRevascularization of transplanted adipose tissue:a study in the dorsal skinfold chamber of hamsters Ann Plast Surg 48 53–59

    Google Scholar 

  19. JD Latoni DM Marshall A Wolfe (2000) ArticleTitleOvergrowth of fat autotransplanted for correction of localized steroid-induced atrophy Plast Reconstr Surg 106 1566–1569

    Google Scholar 

  20. AD Mandrekas GJ Zambacos C Kittas (1998) ArticleTitleCyst formation after fat injection Plast Reconstr Surg 102 1708–1709

    Google Scholar 

  21. R Moscona O Shoshani H Lichtig E Karnielli (1994) ArticleTitleViability of adipose tissue injected and treated by different methods: an experimental study in the rat Ann Plast Surg 33 500–506

    Google Scholar 

  22. A Nguyen KA Pasyk TN Bouvier CA Hasset LC Argenta (1990) ArticleTitleComparative study of survival of autologous adipose tissue taken and transplanted by differtent techniques Plast Reconstr Surg 85 378–386

    Google Scholar 

  23. I Niechajev O Sevcuk (1994) ArticleTitleLong-term results of fat transplantation: clinical and histologic studies Plast Reconstr Surg 94 496–506 Occurrence Handle1:STN:280:ByuA38zksVA%3D Occurrence Handle8047602

    CAS  PubMed  Google Scholar 

  24. T Nishimura H Hashimoto I Nakanishi M Furukawa (2000) ArticleTitleMicrovascular angiogenesis and apopitosis in the survival of free fat grafts Laryngoscope 110 1333–1338

    Google Scholar 

  25. O’Tuama LA, Bjornson B, Chugani H, Treves ST: 1994 Brain. In: Treves ST (ed) Pediatric nuclear medicine, 2nd ed. New York: Springer-Verlag

  26. L Peer (1950) ArticleTitleLoss of weight and volume in human fat grafts with postulation of cell survive theory Plast Reconstr Surg 5 217–230

    Google Scholar 

  27. PA Perèn JB Gomez J Guerrerosantos CA Salazar (2000) ArticleTitleGluteus augmentation with fat grafting Aesth Plast Surg 24 412–417 Occurrence Handle10.1007/s002660010069

    Article  Google Scholar 

  28. J Smahel (1989) ArticleTitleExperimental implantation of adipose tissue fragments Br J Plast Surg 42 207–211

    Google Scholar 

  29. M Stampos P Xepoulias (2001) ArticleTitleFat transplantation for soft tissue augmentation in the lower limbs Aesth Plast Surg 25 256–261

    Google Scholar 

  30. Y Ullmann M Hyams Y Ramon D Beach IJ Peled ES Lindenbaum (1998) ArticleTitleEnhancing the survival of aspirated human fat injected into nude mice Plast Reconstr Surg 101 1940–1944

    Google Scholar 

  31. AM Verbruggen (1998) Lipophilic tracers for the study of regional cerebral blood flow IPC Murray PJ Ell (Eds) Nuclear medicine in clinical diagnosis and treatment, vol 1 Churchill Livingstone London 499–520

    Google Scholar 

  32. F Viterbo M Marquest M Valente (1994) ArticleTitleFat tissue injection versus graft: experimental study in rabbits Ann Plast Surg 33 184–192

    Google Scholar 

Download references

Acknowledgments

This article was supported by the Trakya University Research Fund.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Trakya University, Edirne, Turkey

    A. Cemal Aygit M.D., Husamettin Top M.D., Beyhan Cakir M.D. & M. Fatih Firat M.D.

  2. Department of Nuclear Medicine, Trakya University, Edirne, Turkey

    Ali Sarikaya M.D.

  3. Department of Pathology, Trakya University, Edirne, Turkey

    Latife Doganay M.D.

  4. Trakya Universitesi Tip Fakultesi, Plastik ve Rekonstruktif Cerrahi AD, 22030, Edirne, Turkey

    A. Cemal Aygit M.D.

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  1. A. Cemal Aygit M.D.
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  2. Ali Sarikaya M.D.
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  3. Latife Doganay M.D.
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  4. Husamettin Top M.D.
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  5. Beyhan Cakir M.D.
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  6. M. Fatih Firat M.D.
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Correspondence to A. Cemal Aygit M.D..

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Aygit , A.C., Sarikaya, A., Doganay, L. et al. The Fate of Intramuscularly Injected Fat Autografts: An Experimental Study in Rabbits. Aesth Plast Surg 28, 334–339 (2004). https://doi.org/10.1007/s00266-004-3121-7

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  • DOI: https://doi.org/10.1007/s00266-004-3121-7

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