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Up-regulation expressions of lysyl oxidase family in Anterior Cruciate Ligament and Medial Collateral Ligament fibroblasts induced by Transforming Growth Factor-Beta 1

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Abstract

Purpose

The lysyl oxidase (LOX) family plays a crucial role in the formation and stabilisation of extracellular matrix (ECM) by catalysing the cross-linking of collagen and elastin, implicating its important fundamental roles in injury healing. A high level of transforming growth factor-β1 (TGF-β1) accompanies the inflammatory phase of an injury of the knee joint. Our purpose was to detect the expressions of the LOX family in anterior cruciate ligament (ACL) and medial collateral ligament (MCL) response to TGF-β1.

Methods

This study used reversed transcript PCR, real time quantitative PCR and Western blot for analyses.

Results

The results showed significant increases in mRNA levels of LOX family members. At 5 ng/ml concentration of TGF-β1, the gene profiles of LOXs showed most active, and LOX and LOXL-3 showed increasing peaks at 12 hours after TGF-β1 treatment (LOX: 7.2, 8.8-fold and LOXL-3: 3.8, 5.4-fold compared with normal controls in ACL and MCL, respectively); LOXL-1, LOXL-2 and LOXL-4 reached their highest amounts at six hours (LOXL-1: 1.9, 2.4-fold; LOXL-2: 14.8, 16.2-fold; LOXL-4: 2.5, 4.4-fold in ACL and MCL, respectively). Protein assays revealed that LOXs in ACL cells had relatively lower response to TGF-β1 compared with those in MCL cells.

Conclusions

The differential expression and activities of LOXs might help to explain the intrinsic difference between ACL and MCL, and LOXs could imply a potential capability for ACL healing.

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Acknowledgements

This study was supported by Project 111 (B06023, China), an OREF Award (USA) and by NIH AR45635 (USA).

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Authors and Affiliations

  1. “111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, China

    Jing Xie, Jiahuan Jiang, Yanjun Zhang, ChunMing Xu, Lin Yin, Chunli Wang & K. L. Paul Sung

  2. Departments of Bioengineering and Orthopaedics, University of California, 9500 Gilman Drive, La Jolla, San Diego, CA, 92093-0412, USA

    Peter C. Y. Chen & K. L. Paul Sung

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  1. Jing Xie
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  2. Jiahuan Jiang
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  3. Yanjun Zhang
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  4. ChunMing Xu
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  5. Lin Yin
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  6. Chunli Wang
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  7. Peter C. Y. Chen
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  8. K. L. Paul Sung
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Correspondence to K. L. Paul Sung.

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Xie, J., Jiang, J., Zhang, Y. et al. Up-regulation expressions of lysyl oxidase family in Anterior Cruciate Ligament and Medial Collateral Ligament fibroblasts induced by Transforming Growth Factor-Beta 1. International Orthopaedics (SICOT) 36, 207–213 (2012). https://doi.org/10.1007/s00264-011-1261-3

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  • DOI: https://doi.org/10.1007/s00264-011-1261-3

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