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Hydroxyapatite coating enhances polyethylene terephthalate artificial ligament graft osseointegration in the bone tunnel

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Abstract

The purpose of this study was to investigate whether hydroxyapatite (HAp) coating could induce polyethylene terephthalate (PET) artificial ligament graft osseointegration in the bone tunnel. Twenty-four New Zealand white rabbits underwent artificial ligament graft transplantation in bilateral proximal tibia tunnels. One limb was implanted with HAp-coated PET graft, and the contralateral limb was implanted with non-HAp-coated PET graft as control. The rabbits were randomly sacrificed at four and eight weeks after surgery. The loads to failure of the experimental group at eight weeks were significantly higher than those of the control group (p = 0.0057). Histologically, application of HAp coating induced new bone formation between graft and bone at eight weeks compared with the controls. Real-time polymerase chain reaction examination revealed significantly elevated messenger ribonucleic acid expression levels of osteopontin and collagen I in the grafts of the HAp group compared with the controls at four weeks (p < 0.05). The study has shown that HAp coating on the PET artificial ligament surface has a positive effect in the induction of artificial ligament osseointegration within the bone tunnel.

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Acknowledgments

This project was subsidised by the 863 Project (No. 2007AA021902, 2007AA021903) and 973 Project (No. 2009CB930000) from the Ministry of Science and Technology of China. Many thanks to Dr. Shangchun Guo and Dr. Jianying Shi for their help with histological examinations.

Conflict of interest

The authors report no conflict of interest.

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Authors and Affiliations

  1. Department of Sports Medicine, Huashan Hospital, Shanghai, People’s Republic of China

    Hong Li, Yunsheng Ge, Yang Wu, Jia Jiang, Kai Gao & Shiyi Chen

  2. Shanghai Research Center of Biomedical Engineering, Shanghai, People’s Republic of China

    Pengyun Zhang & Lingxiang Wu

  3. No 12, Wulumuqi Zhong Road, Shanghai, 200040, China

    Shiyi Chen

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  1. Hong Li
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  2. Yunsheng Ge
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  3. Yang Wu
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  4. Jia Jiang
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  5. Kai Gao
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  6. Pengyun Zhang
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  7. Lingxiang Wu
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  8. Shiyi Chen
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Correspondence to Shiyi Chen.

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Li, H., Ge, Y., Wu, Y. et al. Hydroxyapatite coating enhances polyethylene terephthalate artificial ligament graft osseointegration in the bone tunnel. International Orthopaedics (SICOT) 35, 1561–1567 (2011). https://doi.org/10.1007/s00264-010-1158-6

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  • DOI: https://doi.org/10.1007/s00264-010-1158-6

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