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Proteomic analysis of mineralising osteoblasts identifies novel genes related to bone matrix mineralisation

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Abstract

Bone matrix mineralisation plays a critical role in the determination of the overall biomechanical competence of bone. However, the molecular mechanisms of bone matrix mineralisation have not been fully elucidated. We used a proteomic approach to identify proteins and genes that may play a role in osteoblast matrix mineralisation. Proteomic differential display revealed a protein band that appeared only in mineralising mouse 7F2 osteoblasts. In-gel protein digestion and mass spectrometry proteomic analysis of this protein band identified 16 proteins. Furthermore, their corresponding transcripts were upregulated. This identification of proteins that may be associated with bone matrix mineralisation presents important new information toward a better understanding of the precise mechanisms of this process.

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Acknowledgments

The authors would like to thank Melvin Glimcher, Lila Graham and Patrick O’Neill for their critical reading of the manuscript and Marie Torres for her assistance in proteomic analysis. This work was supported by grants from The Peabody Foundation to Melvin J. Glimcher.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Harvard Medical School, Children’s Hospital Boston, 300 Longwood Avenue, Boston, MA, 02115, USA

    Fawzy A. Saad

  2. Department of Orthopaedics, Vienna General Hospital, Medical University of Vienna, Vienna, Austria

    Jochen G. Hofstaetter

  3. Ludwig Boltzmann Institute of Osteology at Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 4th Medical Department, Hanusch Hospital, Vienna, Austria

    Jochen G. Hofstaetter

Authors
  1. Fawzy A. Saad
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  2. Jochen G. Hofstaetter
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Correspondence to Fawzy A. Saad.

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Saad, F.A., Hofstaetter, J.G. Proteomic analysis of mineralising osteoblasts identifies novel genes related to bone matrix mineralisation. International Orthopaedics (SICOT) 35, 447–451 (2011). https://doi.org/10.1007/s00264-010-1076-7

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  • DOI: https://doi.org/10.1007/s00264-010-1076-7

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