Abstract
Platelet-rich plasma (PRP), a platelet concentrate made of autogenous blood, has been used to improve bone and soft tissue defect healing in recent years. The aim of this study was to assess the effect of PRP on articular cartilage defects in a rabbit model. Forty-eight osteochondral defects created in the femoropatellar groove were (a) left untreated, (b) treated with autogenous PRP in a poly-lactic-glycolic acid (PLGA), or (c) with PLGA alone. Platelets were enriched 5.12-fold compared to normal blood in the PRP. After four and 12 weeks, the explanted tissue specimens were assessed by macroscopic examination, micro-computed tomography, and histological evaluation. Macroscopic examination, micro-computed tomography and histology of the newly formed cartilage and bone in the defect differ significantly between the PRP-treated and the untreated groups, and stimulatory effect of PRP on osteochondral formation was observed. In conclusion, PRP in PLGA improves osteochondral healing in a rabbit model.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akeda K, An HS, Okuma M, Attawia M, Miyamoto K, Thonar EJ, et al. (2006) Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis. Osteoarthr Cartil 14:1272–1280
Boopalan PR, Sathishkumar S, Kumar S, Chittaranjan S (2006) Rabbit articular cartilage defects treated by allogenic chondrocyte transplantation. Int Orthop 30:357–361
Borovecki F, Pecina Slaus N, Vukicevic S (2007) Biological mechanisms of bone and cartilage remodelling-genomic perspective. Int Orthop 31:799–805
Buckwalter JA, Mankin HA (1998) Articular cartilage repair and transplantation. Arthritis Rheum 41:1331–1342
Chubinskaya S, Hurtig M, Rueger DC (2007) OP-1/BMP-7 in cartilage repair. Int Orthop 31:773–781
Drengk A, Zapf A, Stürmer EK, Stürmer KM, Frosch KH (2008) Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells. Cells Tissues Organs. Aug 11. [Epub ahead of print]
Evans CH, Palmer GD, Pascher A et al (2007) Facilitated endogenous repair: making tissue engineering simple, practical, and economical. Tissue Eng 13:1987–1993
Farrag TY, Lehar M, Cerhaegen P, Carson KA, Byrne PJ (2007) Effect of platelet rich plasma and fibrin sealant on facial nerve regeneration in a rat model. Laryngoscope 117:157–165
Gotterbarm T, Richter W, Jung M, Berardi Vilei S, Mainil-Varlet P, Yamashita T (2006) An in vivo study of a growth-factor enhanced, cell free, two-layered collagen-tricalcium phosphate in deep osteochondral defects. Biomaterials 27:3387–3395
Holland TA, Bodde EW, Baggett LS, Tabata Y, Mikos AG, Jansen JA (2005) Osteochondral repair in the rabbit model utilizing bilayered, degradable oligo(poly(ethylene glycol) fumarate) hydrogel scaffolds. J Biomed Mater Res A 75:156–167
Ishida K, Kuroda R, Miwa M, Tabata Y, Hokugo A, Kawamoto T (2007) The regenerative effects of platelet-rich plasma on meniscal cells in vitro and its in vivo application with biodegradable gelatin hydrogel. Tissue Eng 13:1103–1112
Jelic M, Pecina M, Haspl M et al (2001) Regeneration of articular chondral defects by osteogenic protein-1 (bone morphogenetic protein-7) in sheep. Growth Factors 19:101–113
Kim SH, Yoon SJ, Choi B, Ha HJ, Rhee JM, Kim MS (2006) Evaluation of various types of scaffold for tissue engineered intervertebral disc. Adv Exp Med Biol 585:169–181
Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638–646
Marx RE (2004) Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 62:489–496
Nikolidakis D, Jansen JA (2008) The biology of platelet-rich plasma and its application in oral surgery: literature review. Tissue Eng Part B Rev 14:249–258
Pecina M, Jelic M, Martinovic S et al (2002) Articular cartilage repair: the role of bone morphogenetic proteins. Int Orthop 26:131–136
Peterson L, Minas T, Brittberg M, Lindahl A (2003) Treatment of osteochondritis dissecans of the knee with autologous chondrocyte transplantation: results at two to ten years. J Bone Jt Surg Am 85:17–24
Kasten P, Vogel J, Geiger F, Niemeyer P, Luginbuhl R, Szalay K (2008) The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials 29:3983–3992
Schuckert KH, Jopp S, Teoh SH (2008) Mandibular defect reconstruction using three-dimensional polycaprolactone scaffold in combination with platelet-rich plasma and recombinant human bone morphogenetic protein-2: de novo synthesis of bone in a single case. Tissue Eng Part A. Sep 3. [Epub ahead of print]
Uematsu K, Hattori K, Ishimoto Y, Yamauchi J, Habata T, Takakura Y (2005) Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly lactic-glycolic acid (PLGA) scaffold. Biomaterials 26:4273–4279
Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze-Mosgau S, Zimmermann R (2004) Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. Clin Oral Implants Res 15:187–193
Acknowledgements
This study was supported by the Key Project Foundation of the Ministry of Health of P.R. China.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the linked to the electronic supplementary material
Supplementary 1
(DOC 2748 kb)
Supplementary 2
(DOC 916 kb)
Supplementary 3
(DOC 5194 kb)
Rights and permissions
About this article
Cite this article
Sun, Y., Feng, Y., Zhang, C.Q. et al. The regenerative effect of platelet-rich plasma on healing in large osteochondral defects. International Orthopaedics (SICOT) 34, 589–597 (2010). https://doi.org/10.1007/s00264-009-0793-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00264-009-0793-2