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Regional and cellular localisation of BMPs and their inhibitors’ expression in human fractures

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Abstract

The objective of this study was to determine whether BMP-2 and -14, noggin, and chordin could be detected in human fractures and to assess their regional and cellular distribution. The expression of these proteins was detected by immunohistochemistry in an archive of human fractures. BMP-2 and BMP-14 expression was strongest in areas of cartilage formation and, to a lesser extent, in areas of bone formation. Within areas of cartilage formation, both BMP-2 and BMP-14 were expressed more strongly by the non-hypertrophic chondrocytes. The BMP inhibitors noggin and chordin were also expressed most intensely in areas of cartilage formation and there was no difference in their expression between the non-hypertrophic and hypertrophic chondrocytes. Our study demonstrates the expression of BMP-14 and the BMP inhibitors in human fractures for the first time, and our findings will contribute to an improved understanding of the physiological processes in bone repair.

Résumé

Le but de cette étude est de déterminer si la BMP-2 et la BMP-14 et leurs inhibiteurs noggin et chordin peuvent être détectées dans les fractures chez l’homme, avec une évaluation de leur distribution cellulaire et régionale. Matériels et méthode: l’expression de ces protéines est détectée par immunohistochimie. Résultats: la BMP-2 et la BMP-14 s’expriment de façon importante au niveau de la formation du cartilage et au niveau de la restauration osseuse. Au niveau du cartilage, la BMP-2 et la BMP-14 s’expriment surtout au niveau des chondrocytes non hypertrophiques. Les inhibiteurs de la BMP s’expriment également au niveau de la formation cartilagineuse, il n’y a pas de différence dans leur expression pour les chondrocytes non hyperthrophiques ou hyperthrophiques. En conclusion: notre étude démontre que l’expression de la BMP-14 et de ses inhibiteurs peuvent être détectées dans les fractures chez l’homme. Nos constatations doivent contribuer à une meilleure compréhension du processus de réparation osseuse.

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Acknowledgement

This study was supported by NIH grant AR 050243.

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Authors and Affiliations

  1. Center for Molecular Orthopaedics, Harvard Medical School, 221 Longwood Avenue, Boston, MA, 02115, USA

    Francois N. K. Kwong & Christopher H. Evans

  2. Tissue Injury and Repair Group, School of Clinical and Laboratory Sciences, Faculty of Medical and Human Sciences, The University of Manchester, Stopford Building, Oxford Road, Manchester, M13 9PT, UK

    Francois N. K. Kwong, Judith A. Hoyland & Anthony J. Freemont

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  1. Francois N. K. Kwong
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  2. Judith A. Hoyland
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  4. Anthony J. Freemont
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Correspondence to Francois N. K. Kwong.

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Kwong, F.N.K., Hoyland, J.A., Evans, C.H. et al. Regional and cellular localisation of BMPs and their inhibitors’ expression in human fractures. International Orthopaedics (SICO 33, 281–288 (2009). https://doi.org/10.1007/s00264-008-0691-z

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  • DOI: https://doi.org/10.1007/s00264-008-0691-z

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