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Osteogenic abilities of bone marrow stromal cells are not defective in patients with osteonecrosis

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Abstract

We investigated whether the proliferation activities and osteogenic capacities of bone marrow stromal cells (BMSCs) are depressed in patients with osteonecrosis of the femoral head (ONFH). BMSCs were isolated from fresh bone marrow of the iliac crests of 54 donors and were differentiated into osteogenic lineage in vitro. The results of 27 consecutive patients with ONFH (16 idiopathic and 11 alcohol-induced) were compared with those of 27 patients with a nonnecrotic hip disorder. The proliferative activities of BMSCs in patients with ONFH were not found to be reduced and their osteogenic capacities (alkaline phosphatase activity and calcium deposition amount) were unaltered during in vitro differentiation. Results from patients with idiopathic or alcohol-induced ONFH were similar to those of matched patients with nonnecrotic disorder. These findings suggest that the osteogenic potentials of BMSCs are not defective in patients with ONFH.

Résumé

Nous pensions que la prolifération et l’activité des cellules ostéogéniques de la moelle osseuse (BMSCs) étaient diminuées chez les patients qui présentaient une ostéonécrose de la tête fémorale (ONFH). Matériel et méthodes : Les BMSCs ont été isolées de la moelle fraiche, prélevées sur la crête iliaque de 54 donneurs et classées selon leur lignée ostéogénique. Les résultats de 27 patients avec ostéonécrose, 16 idopathiques et 11 d’origine éthylique ont été comparés à 27 patients ne présentant pas de nécrose de hanche. Résultat : l’activité des cellules de moelle osseuse chez les patients avec ostéonécrose ont été trouvées diminuées et leur capacité ostéogénique (activité phosphatase alkaline et déposition calcique) ne sont pas altérées lors de l’examen in vitro. Résultat : les patients présentant une ostéonécrose idiopathique ou éthylique ont une activité ostéogénique similaire comparée à celle des patients ne présentant pas de troubles d’origine nécrotique.

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Acknowledgements

The authors thank Jeong Hyun Bang, B.S., for technical assistance with the biological assay.

This work was supported by a grant (no. 04-2006-088) from the Seoul National University Hospital Research Fund.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul, South Korea

    Jeong Joon Yoo, Kyung-Hoi Koo, Kang Sup Yoon & Hee Joong Kim

  2. Department of Orthopaedic Surgery, Korea Cancer Center Hospital, Seoul, South Korea

    Won Seok Song

  3. Medical Research Center, Seoul National University, Seoul, South Korea

    Hee Joong Kim

  4. Department of Orthopaedic Surgery, Seoul National University Hospital, 28 Yeongeon-dong, Jongno-gu, 110-744, Seoul, South Korea

    Hee Joong Kim

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  1. Jeong Joon Yoo
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  2. Won Seok Song
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  3. Kyung-Hoi Koo
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  4. Kang Sup Yoon
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  5. Hee Joong Kim
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Correspondence to Hee Joong Kim.

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Each author certifies that his or her institution has approved or waived approval for the human protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.

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Yoo, J.J., Song, W.S., Koo, KH. et al. Osteogenic abilities of bone marrow stromal cells are not defective in patients with osteonecrosis. International Orthopaedics (SICOT) 33, 867–872 (2009). https://doi.org/10.1007/s00264-008-0524-0

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  • DOI: https://doi.org/10.1007/s00264-008-0524-0

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