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The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

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Abstract

The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; α-D-GalpNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.

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Acknowledgments

Authors thank Dr. Mary Kay Pflum for Jurkat and JurkatTAg cell lines. P.R.A acknowledges Wayne State University for financial support and the National Institutes of Health/National Cancer Institute for an R01 CA 156661 award.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Chemistry, Wayne State University, 5100 Cass Avenue, Detroit, MI, 48202, USA

    Ravindra A. De Silva, Dananjaya K. Appulage & Peter R. Andreana

  2. Division of Hematology and Oncology, Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA

    Halina Pietraszkiewicz, Joe Media & Fred A. Valeriote

  3. The Josephine Ford Cancer Center, 440 Burroughs, Room 415, Detroit, MI, 48202, USA

    Halina Pietraszkiewicz, Joe Media & Fred A. Valeriote

  4. Department of Public Health Sciences Henry Ford Hospital, 1 Ford Place, 1D, Detroit, MI, 48202, USA

    Kevin R. Bobbitt

  5. 21st Century Therapeutics, Inc., 52623 Seven Oaks Dr. Shelby Township, Detroit, MI, 48316, USA

    JiaJiu Shaw

Authors
  1. Ravindra A. De Silva
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  2. Dananjaya K. Appulage
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  3. Halina Pietraszkiewicz
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  4. Kevin R. Bobbitt
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  5. Joe Media
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  6. JiaJiu Shaw
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  7. Fred A. Valeriote
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  8. Peter R. Andreana
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Corresponding author

Correspondence to Peter R. Andreana.

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De Silva, R.A., Appulage, D.K., Pietraszkiewicz, H. et al. The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17. Cancer Immunol Immunother 61, 581–585 (2012). https://doi.org/10.1007/s00262-012-1205-9

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  • DOI: https://doi.org/10.1007/s00262-012-1205-9

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