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The European searchable tumour line database

  • Focussed Research Review
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Abstract

The European Searchable Tumour line Database (ESTDAB) (http://www.ebi.ac.uk/ipd/estdab) is a freely available and fully searchable database of melanoma-derived cell lines, which have been characterised for over 250 immunologically relevant markers by a consortium of European scientists. The database is linked to a cell bank, which can provide melanoma cell lines to non-profit investigators for a nominal handling charge. All cells are fully HLA typed at the genomic and surface expression levels. The expression of a number of surface antigens, apoptotic markers, tumour-associated antigens and extracellular matrix proteins has also been determined. Cytokine secretion has been tested and polymorphisms in cytokine genes have been identified. Glycans at the cell surface were identified and glycosyltransferase activity quantified. Cell lines with a particular constellation of these parameters can be sought online via the ESTDAB interface, which is included as part of the Immuno-Polymorphism Database (IPD) section of the European Bioinformatics Institute’s (EBI) website.

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References

  1. Pawelec G, Marsh SGE (2006) ESTDAB: a collection of immunologically characterised melanoma cell lines and searchable databank. Cancer Immunol Immunother 55:623–627

    Article  PubMed  CAS  Google Scholar 

  2. Arguello JR, Little AM, Bohan E, Goldman JM, Marsh SGE, Madrigal JA (1998) High resolution HLA class I typing by reference strand mediated conformation analysis (RSCA). Tissue Antigens 52:57–66

    Article  PubMed  CAS  Google Scholar 

  3. Arguello JR, Little AM, Pay AL, Gallardo D, Rojas I, Marsh SGE, Goldman JM, Madrigal JA (1998) Mutation detection and typing of polymorphic loci through double-strand conformation analysis. Nat Genet 18:192–194

    Article  PubMed  CAS  Google Scholar 

  4. Rozemuller EH, Bouwens AG, van Oort E, Versluis LF, Marsh SGE, Bodmer JG, Tilanus MG (1995) Sequencing-based typing reveals new insight in HLA-DPA1 polymorphism. Tissue Antigens 45:57–62

    Article  PubMed  CAS  Google Scholar 

  5. Santamaria P, Lindstrom AL, Boyce-Jacino MT, Myster SH, Barbosa JJ, Faras AJ, Rich SS (1993) HLA class I sequence-based typing. Hum Immunol 37:39–50

    Article  PubMed  CAS  Google Scholar 

  6. thor Straten P, Guldberg P, Gronbaek K, Hansen MR, Kirkin AF, Seremet T, Zeuthen J, Becker JC (1999) In situ T cell responses against melanoma comprise high numbers of locally expanded T cell clonotypes. J Immunol 163:443–447

    PubMed  CAS  Google Scholar 

  7. Robinson J, Malik A, Parham P, Bodmer JG, Marsh SGE (2000) IMGT/HLA database—a sequence database for the human major histocompatibility complex. Tissue Antigens 55:280–287

    Article  PubMed  CAS  Google Scholar 

  8. Robinson J, Waller MJ, Parham P, Bodmer JG, Marsh SGE (2001) IMGT/HLA database—a sequence database for the human major histocompatibility complex. Nucleic Acids Res 29:210–213

    Article  PubMed  CAS  Google Scholar 

  9. Robinson J, Waller MJ, Stoehr P, Marsh SGE (2005) IPD—the immuno polymorphism database. Nucleic Acids Res 33:D523–D526

    Article  PubMed  CAS  Google Scholar 

  10. Laidler P, Litynska A, Hoja-Lukowicz D, Labedz M, Przybylo M, Ciolczyk-Wierzbicka D, Pochec E, Trebacz E, Kremser E (2006) Characterization of glycosylation and adherent properties of melanoma cell lines. Cancer Immunol Immunother 55:112–118

    Article  PubMed  CAS  Google Scholar 

  11. Rodriguez T, Mendez R, Del Campo A, Jimenez P, Aptsiauri N, Garrido F, Ruiz-Cabello F (2007) Distinct mechanisms of loss of IFN-gamma mediated HLA class I inducibility in two melanoma cell lines. BMC Cancer 7:34

    Article  PubMed  Google Scholar 

  12. Mendez R, Rodriguez T, Del Campo A, Monge E, Maleno I, Aptsiauri N, Jimenez P, Pedrinaci S, Pawelec G, Ruiz-Cabello F, Garrido F (2008) Characterization of HLA class I altered phenotypes in a panel of human melanoma cell lines. Cancer Immunol Immunother 57:719–729

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This project was supported by the European Commission within the Fifth Framework Infrastructures Program (contract no. QLRI-CT-2001-01325). ESTDAB was a continuation of EUCAPS (European Concerted Action on Peptide Sensitization. http://www.medizin.uni-tuebingen.de/eucaps/home/). The characterisation of the lines used in ESTDAB was provided by Raja Choudhury, Federico Garrido, Rolf Kiessling, Piotr Laidler, Elissaveta Naumova, Per thor Straten and Wolfgang Wagner.

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Authors and Affiliations

  1. Anthony Nolan Research Institute, Royal Free Hospital, Pond Street, Hampstead, London, NW3 2QG, UK

    James Robinson, Chrissy h. Roberts, I. Anthony Dodi, J. Alejandro Madrigal & Steven G. E. Marsh

  2. UCL Cancer Institute, Department of Haematology, University College London, Royal Free Campus, Pond Street, Hampstead, London, NW3 2QG, UK

    Chrissy h. Roberts, I. Anthony Dodi, J. Alejandro Madrigal & Steven G. E. Marsh

  3. Zentrum für Medizinische Forschung, University of Tübingen Medical School, Waldhörnlestr. 22, 72072, Tübingen, Germany

    Graham Pawelec & Lilly Wedel

Authors
  1. James Robinson
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  2. Chrissy h. Roberts
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  3. I. Anthony Dodi
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  4. J. Alejandro Madrigal
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  5. Graham Pawelec
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  6. Lilly Wedel
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  7. Steven G. E. Marsh
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Corresponding author

Correspondence to Steven G. E. Marsh.

Additional information

This paper is a focussed research review from the meeting which took place on the 28th–29th May 2008 in Nottingham, UK, celebrating the contribution of Prof. I.A. “Tony” Dodi (29.1.2008) to the EU project “Network for the identification and validation of antigens and biomarkers in cancer and their application in clinical tumour immunology (ENACT).”

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Robinson, J., Roberts, C.h., Dodi, I.A. et al. The European searchable tumour line database. Cancer Immunol Immunother 58, 1501–1506 (2009). https://doi.org/10.1007/s00262-008-0656-5

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  • DOI: https://doi.org/10.1007/s00262-008-0656-5

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