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Low representation of Fc receptor-like 1–5 molecules in leukemic cells from Iranian patients with acute lymphoblastic leukemia

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Abstract

Recent studies have demonstrated expression of Fc receptor-like (FCRL) molecules, a newly identified family with preferential B-cell lineage expression, in some chronic B-cell leukemias with possible implication for classification and/or targeted immunotherapy. In this study, the expression pattern of FCRL1-5 genes was studied in 73 Iranian ALL patients and 35 normal subjects using semi-quantitative RT-PCR method. FCRL protein expression was also investigated by flow cytometry. Our results indicate significant down-regulation of all FCRL genes in ALL compared to normal subjects. Although, FCRL mRNA expression was almost exclusively confined to normal isolated B-cells compared to T-cells, but these genes were similarly expressed in B-ALL, T-ALL and different B-ALL immunophenotypic subtypes. Surface protein expression of FCRL1, 2, 4, and 5 molecules in 10 ALL and 5 normal samples confirmed the PCR results. Expression profile of FCRL molecules in different subtypes of ALL argues against their potential implication as suitable targets for classification and/or immunotherapy of ALL.

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Acknowledgments

This study was supported in part by grants from Tehran University of Medical Sciences and the Food and Drug Administration of the Ministry of Health and Medical Education of Iran.

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Authors and Affiliations

  1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, 14155, Tehran, Iran

    Tohid Kazemi, Hossein Asgarian-Omran, Ali Memarian, Mahdi Shabani & Fazel Shokri

  2. Clinic of Hematology and Oncology, Vali-Asr Hospital, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Ramazan A. Sharifian

  3. Clinic of Hematology, Ali-Asghar Hospital, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Parvaneh Vossough

  4. Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Bita Ansaripour

  5. Immune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden

    Hodjatallah Rabbani

  6. Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran

    Fazel Shokri

  7. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

    Fazel Shokri

Authors
  1. Tohid Kazemi
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  2. Hossein Asgarian-Omran
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  3. Ali Memarian
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  4. Mahdi Shabani
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  5. Ramazan A. Sharifian
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  6. Parvaneh Vossough
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  7. Bita Ansaripour
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  8. Hodjatallah Rabbani
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  9. Fazel Shokri
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Correspondence to Fazel Shokri.

Additional information

T. Kazemi, H. Asgarian-Omran and A. Memarian have contributed equally to this study.

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Kazemi, T., Asgarian-Omran, H., Memarian, A. et al. Low representation of Fc receptor-like 1–5 molecules in leukemic cells from Iranian patients with acute lymphoblastic leukemia. Cancer Immunol Immunother 58, 989–996 (2009). https://doi.org/10.1007/s00262-008-0589-z

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  • DOI: https://doi.org/10.1007/s00262-008-0589-z

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