Abstract
The collapsin response mediator protein 5 (CRMP5) antibody is usually associated with paraneoplastic neurological syndrome (PNS) and small-cell lung cancer (SCLC) or thymoma. The objective of this study was to assess the frequency of CRMP5 antibodies in patients with such tumours and to see if the presence of antibodies was associated with prognosis in these cancers. A multi-well adapted immunoprecipitation assay using radiolabelled recombinant CRMP5 protein, produced by coupled in vitro transcription/translation, was used for the detection of CRMP5 antibodies. Sera from 200 patients with SCLC, 73 patients with thymoma and myasthenia gravis (MG) and from 300 healthy blood donors were examined for CRMP5 antibodies. Positive sera were also examined by immunofluorescence and immune blots. The serological results were compared with disease severity of the patients with thymoma or SCLC. CRMP5 antibodies were detected in 10/200 (5%) of the SCLC, 9/73 (12%) of the thymomas and in 2/300 (0.6%) of the healthy controls by immunoprecipitation. The antibodies were less frequently detected by immunofluorescence or immune blots. There was no significant correlation between CRMP5 antibodies and disease severity. CRMP5 antibodies are more than twice as frequent, and the antibody levels are higher in patients with thymoma and MG than in patients with SCLC. The antibodies are correlated to these tumours, but not to disease severity.
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Acknowledgments
The authors thank Cecilie Totland, Mette Haugen and Kibret Mazengia for technical assistance. We also wish to tank professor Jerome Honnorat, Hospital Neurologique, Lyon, France for kindly providing us with a CRMP5 plasmid and purified recombinant CRMP5 protein, Professor Amelia Evoli, Institute of Neurology, Catholic University, Rome, Italy and Dr Janzen, Neurologische Universität Klinik, Hamburg, Germany for providing some of the thymoma patient sera. This study was supported by grants from Haukeland University Hospital and the University of Bergen, Norway.
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Monstad, S.E., Drivsholm, L., Skeie, G.O. et al. CRMP5 antibodies in patients with small-cell lung cancer or thymoma. Cancer Immunol Immunother 57, 227–232 (2008). https://doi.org/10.1007/s00262-007-0369-1
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DOI: https://doi.org/10.1007/s00262-007-0369-1