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MIP3 alpha stimulates the migration of Langerhans cells in models of human papillomavirus (HPV)-associated (pre)neoplastic epithelium

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Abstract

Although human papillomavirus (HPV) DNA is detected in the majority of cervical cancers and their precursors (squamous intraepithelial lesions; SIL), the persistence or progression of cervical lesions could be associated with quantitative and functional alterations of dendritic/Langerhans cells (DC/LC). As LC abnormalities have been associated with a decreased expression of macrophage inflammatory protein 3α (MIP3α) in cervical SIL, we tested the effect of exogenous MIP3α on the migration of LC in a (pre)neoplastic epithelium formed in vitro. By using a Boyden chamber assay, we first showed that the migratory capacity of LC generated in vitro is significantly increased in the presence of MIP3α compared to control medium. We next demonstrated that MIP3α is able to increase the 3D infiltration of LC in organotypic cultures of HPV-transformed keratinocytes. This property to stimulate LC migration was not altered after inclusion of MIP3α in a bioadhesive polycarbophil gel. Moreover, the function of DC to exert cytostatic effects and to present alloantigens was not altered in the presence of MIP3α.

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Acknowledgments

This work was supported by the IAP (interuniversity attraction pole network P5/31), the CRIV (Centre de Recherche Interuniversitaire en Vaccinologie) with a grant from the Walloon Region and GlaxoSmithKline, the Belgian Fund for Medical Scientific Research and the Centre Anti-Cancereux près l’Université de Liège. P. Delvenne is a Senior Research Associate of the Belgian National Fund for Scientific Research.

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Authors and Affiliations

  1. Department of Pathology, CRCE, B35, University of Liege, CHU Sart Tilman, 4000, Liege, Belgium

    Ludivine Herman, Pascale Hubert, Jean-Hubert Caberg, Jacques Boniver & Philippe Delvenne

  2. Department of Pharmaceutical Technology, CRCE, B35, University of Liege, CHU Sart Tilman, 4000, Liege, Belgium

    Brigitte Evrard

  3. Department of Gynecology and Obstetrics, Karol Marcinkowski School of Medecine, Poznan, Poland

    Witold Kedzia

Authors
  1. Ludivine Herman
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  2. Pascale Hubert
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  3. Jean-Hubert Caberg
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  4. Brigitte Evrard
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  5. Witold Kedzia
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  6. Jacques Boniver
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  7. Philippe Delvenne
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Corresponding author

Correspondence to Philippe Delvenne.

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P. Hubert and L. Herman contributed equally to this work.

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Herman, L., Hubert, P., Caberg, JH. et al. MIP3 alpha stimulates the migration of Langerhans cells in models of human papillomavirus (HPV)-associated (pre)neoplastic epithelium. Cancer Immunol Immunother 56, 1087–1096 (2007). https://doi.org/10.1007/s00262-006-0255-2

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  • DOI: https://doi.org/10.1007/s00262-006-0255-2

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