Comparative ^99mTc-MIBI, ^99mTc-tetrofosmin and ^99mTc-furifosmin uptake in human soft tissue sarcoma cell lines

Abstract.

The uptake characteristics of technetium-99m hexakis-2-methoxyisobutylisonitrile (MIBI), ^99mTc-tetrofosmin and ^99mTc-furifosmin in human soft tissue sarcoma cell lines were investigated and compared. After 10–120 min of incubation at 37°C, 32°C and 22°C with ^99mTc-MIBI, ^99mTc-tetrofosmin and ^99mTc-furifosmin, the kinetics of cellular uptake of these tracers in human soft tissue sarcoma cells SW 684 (fibrosarcoma), SW 872 (liposarcoma), SW 982 (synovial sarcoma) and SW 1353 (chondrosarcoma) was assessed. The uptake of ^99mTc-MIBI, ^99mTc-tetrofosmin and ^99mTc-furifosmin was temperature dependent. The kinetics of uptake of ^99mTc-MIBI and of ^99mTc-tetrofosmin was similar between fibrosarcoma and liposarcoma cells, as well as between synovial sarcoma and chondrosarcoma cells. ^99mTc-furifosmin showed similar uptake kinetics in all cell lines. The uptake of ^99mTc-furifosmin was, however, significantly higher in liposarcoma than in the other cells. The data indicate that the cellular uptake of ^99mTc-MIBI, ^99mTc-tetrofosmin and ^99mTc-furifosmin is dependent on cellular metabolic activity.