Abstract
Purpose
In vivo quantification of radiopharmaceuticals has great potential as a tool in developing new drugs. We investigated the accuracy of in vivo quantification with multi-pinhole single-photon emission computed tomography (SPECT) in rats.
Methods
Fifteen male Lewis rats with different stages of renal dysfunction were injected with 50 MBq 99mTc-dimercaptosuccinic acid. Four to six hours after injection, SPECT of the kidneys was acquired with a new four-headed multi-pinhole collimator camera. Immediately after imaging the rats were sacrificed and the kidneys were counted in a gamma-counter to determine the absorbed activity. SPECT data were reconstructed iteratively and regions of interest (ROIs) were drawn manually. The absolute activity in the ROIs was determined.
Results
Uptake values ranging from 0.71% to 21.87% of the injected activity were measured. A very strong linear correlation was found between the determined activity in vivo and ex vivo (r 2=0.946; slope m=1.059).
Conclusion
Quantification in vivo using this multi-pinhole SPECT system is highly accurate.
References
Habraken JBA, de Bruin K, Shehata M, Booij J, Bennink R, van Eck Smit BL, et al. Evaluation of high-resolution pinhole SPECT using a small rotating animal. J Nucl Med 2001;42:1863–1869
Kwekkeboom DJ, Mueller-Brand J, Paganelli G, Anthony LB, Pauwels S, Kvols LK, et al. Overview of results of peptide receptor radionuclide therapy with 3 radiolabeled somatostatin analogs. J Nucl Med 2005;46:62S–66S
Forrer F, Uusijärvi H, Storch D, Maecke HR, Mueller-Brand J. Treatment with 177Lu-DOTATOC of patients with relapse of neuroendocrine tumors after treatment with 90Y-DOTATOC. J Nucl Med 2005;46:1310–1316
Behr TM, Sharkey RM, Sgouros G, Blumenthal RD, Dunn RM, Kolbert K, et al. Overcoming the nephrotoxicity of radiometal-labeled immunoconjugates. Cancer 1997;80(12 Suppl):2591–2610
Kabasakal L, Turkmen C, Ozmen O, Alan N, Onsel C, Uslu I. Is furosemide administration effective in improving the accuracy of determination of differential renal function by means of technetium-99m DMSA in patients with hydronephrosis. Eur J Nucl Med Mol Imaging 2002;29:1433–1437
Acton PD, Kung HF. Small animal imaging with high resolution single photon emission tomography. Nucl Med Biol 2003;30:889–895
Beekman FJ, van der Have F, Vastenhouw B, van der Linden AJ, van Rijk PP, Burbach JP, et al. U-SPECT-I: a novel system for submillimeter-resolution tomography with radiolabeled molecules in mice. J Nucl Med 2005;46:1194–1200
Lackas C, Schramm NU, Hoppin JW, Engeland U, Wirrwar A, Halling. T-SPECT: a novel imaging technique for small animal imaging. IEEE Trans Nucl Sci 2005;52:181–187
Constantinesco A, Choquet P, Monassier L, Israel-Jost V, Mertz L. Assessment of left ventricular perfusion, volumes, and motion in mice using pinhole gated SPECT. J Nucl Med 2005;46:1005–1011
Acton PD, Choi SR, Plossl K, Kung HF. Quantification of dopamine transporters in the mouse brain using ultra-high resolution single-photon emission tomography. Eur J Nucl Med Mol Imaging 2002;29:691–698
Walrand S, Jamar F, de Jong M, Pauwels S. Evaluation of novel whole-body high-resolution rodent SPECT (Linoview) based on direct acquisition of linogram projections. J Nucl Med 2005;46:1872–1880
Kwekkeboom DJ, Bakker WH, Kooij PP, Konijnenberg MW, Srinivasan A, Erion JL, et al. [177Lu-DOTA0Tyr3]octreotate: comparison with [111In-DTPA0]octreotide in patients. Eur J Nucl Med 2001;28:1319–1325
Acknowledgements
Support was provided by the Swiss National Science Foundation, the Novartis Foundation (Switzerland), the Dutch Organisation for Scientific Research (ZonMw) (the Netherlands) and the Alexander von Humboldt Foundation. The authors wish to thank all supporting personnel of the Department of Nuclear Medicine, Erasmus MC, Rotterdam, and especially Marleen Melis, for their expert help and effort.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Forrer, F., Valkema, R., Bernard, B. et al. In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals. Eur J Nucl Med Mol Imaging 33, 1214–1217 (2006). https://doi.org/10.1007/s00259-006-0178-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00259-006-0178-3