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Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals

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Abstract.

It was reported that Pleurotus ostreatus was transformed unstably using recombinant plasmids containing a hygromycin B phosphotransferase gene (hph) under the control of Aspergillus nidulans expression signals, and that the plasmids were maintained extrachromosomally in the transformants. Here we report a stable and integrative transformation of the fungus to hygromycin B resistance, using a recombinant hph fused with Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase expression signals. Restriction-enzyme-mediated integration (REMI) was also tried and increased the transformation efficiency about ten-fold.

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Authors and Affiliations

  1. Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto, 611-0011 Japan, , , , ,

    T. Irie,  Y. Honda,  T. Watanabe &  M. Kuwahara

  2. Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, Japan, , , , ,

    T. Hirano,  T. Sato &  H. Enei

Authors
  1. T. Irie
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  2. Y. Honda
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  3. T. Hirano
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  4. T. Sato
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  5. H. Enei
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  6. T. Watanabe
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  7. M. Kuwahara
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Irie, .T., Honda, .Y., Hirano, .T. et al. Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals. Appl Microbiol Biotechnol 56, 707–709 (2001). https://doi.org/10.1007/s002530100651

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  • DOI: https://doi.org/10.1007/s002530100651

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