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Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble

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Abstract.

We have developed a stable, DNA-mediated transformation system for the white-rot basidiomycete Trametes versicolor based on the dominant selectable marker shble (phleomycin resistance). We employed a vector containing the selectable marker under control of expression sequences from the basidiomycete Schizophyllum commune and a polyethylene glycol/CaCl2 protoplast-fusion technique to introduce the transforming DNA. This transformation system generated stable phleomycin-resistant transformants at a frequency of four to seven transformants/µg of transforming DNA.

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Authors and Affiliations

  1. Department of Biological Sciences, Central Connecticut State University, 1615 Stanley St., New Britain, CT 06050, USA, , , , ,

    K. Bartholomew

  2. US Biotek, 13725 Lake City Way NE, Seattle, WA, 98125, USA, , , , ,

    G. Dos Santos

  3. Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, SK. S7N 0W9, Canada, , , , ,

    T. Dumonceaux

  4. Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada, , , , ,

    T. Charles

  5. Pulp and Paper Research Institute of Canada, 570 St. John's Boulevard, Pointe Claire QC H9R 3J9, Canada, , , , ,

    F. Archibald

Authors
  1. K. Bartholomew
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  2. G. Dos Santos
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  3. T. Dumonceaux
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  4. T. Charles
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  5. F. Archibald
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Received revision: 8 January 2001

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Bartholomew, .K., Dos Santos, .G., Dumonceaux, .T. et al. Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble . Appl Microbiol Biotechnol 56, 201–204 (2001). https://doi.org/10.1007/s002530100635

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  • DOI: https://doi.org/10.1007/s002530100635

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