Abstract
The effect of extracellular pH and dissolved oxygen on regulation of the pcbAB gene in P.␣chrysogenum was examined, using Northern analysis and a reporter gene fusion. It was found that ambient pH markedly affected levels of pcbAB mRNA whereas maintenance of dissolved oxygen concentration above 10 % had no detectable effect. The presence of a DNA-binding protein, which binds upstream of the pcbAB translational start codon, was also related to ambient pH. In all fermentations, pcbAB mRNA was most abundant at around the late exponential/early stationary phase of a culture.
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Received: 10 May 1996 / Received revision: 14 October 1996 / Accepted: 25 October 1996
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Chu, YW., Renno, D. & Saunders, G. Extracellular pH affects regulation of the pcbAB gene in Penicillium chrysogenum . Appl Microbiol Biotechnol 47, 250–254 (1997). https://doi.org/10.1007/s002530050922
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DOI: https://doi.org/10.1007/s002530050922