Skip to main content
SpringerLink
Log in

Formation of sulfate and glucoside conjugates of benzo[e]pyrene by Cunninghamella elegans

  • ORIGNAL PAPER
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

 Benzo[e]pyrene is a pentacyclic aromatic hydrocarbon, which, unlike its structural isomer benzo[a]pyrene, is not a potent carcinogen or mutagen. The metabolism of benzo[e]pyrene was studied using the filamentous fungus Cunninghamella elegans ATCC 36112. C. elegans metabolized 65% of the [9, 10, 11, 12-3H]benzo[e]pyrene and unlabeled benzo[e]pyrene added to Sabouraud dextrose broth cultures after 120 h of incubation. Three major metabolites of benzo[e]pyrene were separated by reversed-phase high-performance liquid chromatography. These metabolites were identified by 1H and 13C NMR, UV-visible, and mass spectral analyses as 3-benzo[e]pyrenylsulfate, 10-hydroxy-3-benzo[e]pyrenyl sulfate, and benzo[e]pyrene 3-O-β-glucopyranoside.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Division of Microbiology, National Center for Toxicological Research, HFT-250, Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA. Fax: (501) 543-7307 E-mail: Jpothuluri@nctr.fda.gov, , , , , , US

    J. V. Pothuluri, F. E. Evans, T. M. Heinze & C. E. Cerniglia

Authors
  1. J. V. Pothuluri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. E. Evans
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. M. Heinze
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. E. Cerniglia
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 7 September 1995/Received revision: 14 November 1995/Accepted: 11 December 1995

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pothuluri, J., Evans, F., Heinze, T. et al. Formation of sulfate and glucoside conjugates of benzo[e]pyrene by Cunninghamella elegans . Appl Microbiol Biotechnol 45, 677–683 (1996). https://doi.org/10.1007/s002530050747

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s002530050747

Keywords

Search

Navigation