Abstract
The effects of nutrient amendment and alginate encapsulation on survival of and phenanthrene mineralization by the bioluminescentPseudomonas sp. UG14Lr in creosote-contaminated soil slurries were examined. UG14Lr was inoculated into creosote-contaminated soil slurries either as a free cell suspension or encapsulated in alginate beads prepared with montmorillonite clay and skim milk. Additional treatments were free-cell-inoculated slurries amended with sterile alginate beads, free-cell-inoculated and uninoculated slurries amended with skim milk only, and uninoculated, unamended slurries. Mineralization was determined by measuring14CO2 released from radiolabelled phenanthrene. Survival was measured by selective plating and bioluminescence. Inclusion of skim milk was found to enhance both survival of and phenanthrene mineralization by free and encapsulated UG14Lr cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Acea MJ, Alexander M (1988) Growth and survival of bacteria introduced into carbon-amended soil. Soil Biol Biochem 28:703–709
Bashan Y (1986) Alginate beads as synthetic inoculant carriers for slow release of bacteria that effect plant growth. Appl Environ Microbiol 51:1089–1098
Cassidy MB, Leung K, Lee H, Trevors JT (1995) Survival oflac-lux markedPseudomonas aeruginosa UG2Lr cells encapsulated in κ-carrageenan and alginate. J Microbiol Methods (in press)
De Lorenzo V, Herrero M, Jakubzik U, Timmis KN (1990) Mini-Tn5 transporon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gramnegative eubacteria. J Bacteriol 172:6568–6572
Elsas JD van, Trevors JT, Jain D, Wolters AC, Heijnen CE, Overbeek LS van (1992) Survival of, and root colonization by, alginate-encapsulatedPseudomonas fluorescens cells following introduction into soil. Biol Fertil Soils 14:14–22
England LS, Lee H, Trevors JT (1993) Bacterial survival in soil: effect of clays and protozoa. Soil Biol Biochem 25:525–531
Flemming CA, Leung KT, Lee H, Trevors JT, Greer CW (1994) Survival oflux-lac marked biosurfactant-producingPseudomonas aeruginosa UG2L in soil: monitored by non-selective plating and polymerase chain reaction. Appl Environ Microbiol 60:1606–1613
Heijnen CE, Hok-A-Hin CH, Veen JA van (1992) Improvements to the use of bentonite clay as a protective agent, increasing survival levels of bacteria introduced into soil. Soil Biol Biochem 24:533–538
Herrero M, De Lorenzo V, Timmis KN (1990) Transporon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J Bacteriol 172:6557–6567
Jackman SC, Lee H, Trevors JT (1992) Survival, detection and containment of bacteria. Microb Releases 1:125–154
Kiyohara H, Nagao K, Yano K (1982) Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates. Appl Environ Microbiol 43:454–457
Leung K, Cassidy MB, Holmes SB, Lee H, Trevors JT (1995) Survival of κ-carrageenan-encapsulated and unencapsulatedPseudomonas aeruginosa UG2Lr cells in forest soil monitored by polymerase chain reaction and spread plating. FEMS Microb Ecol (in press)
Paul E, Fages J, Blane P, Goma G, Pareilleux A (1993) Survial of alginate-entrapped cells ofAxospirillum lipoferum during dehydration and storage in relation to water properties. Appl Microbiol Biotechnol 40:34–39
Providenti MA (1994) Effect ofPseudomonas aeruginosa UG2 inocula and biosurfactants on phenanthrene mineralization byPseudomonas sp. UG14. MSc Thesis, Department of Environmental Biology, University of Guelph, Canada
Recorbet G, Steinberg C, Faurie G (1992) Survival in soil of genetically engineeredEscherichia coli as related to inoculum density, predation and competition. FEMS Microbiol Ecol 101:251–260
Trevors JT (1991) Respiratory activity of alginate-encapsulatedPseudomonas fluorescens cells introduced into soil. Appl Microbiol Biotechnol 35:416–419
Trevors JT, Cook S (1992) A comparison of plating media and diluents for enumeration of aerobic bacteria in a loam soil. J Microbiol Methods 14:271–275
Trevors JT, Elsas JD van, Lee H, Overbeek LS van (1992) Use of alginate and other carriers for encapsulation of microbial cells for use in soil. Microb Releases 1:61–69
Trevors JT, Elsas JD van, Lee H, Wolters AC (1993) Survival of alginate-encapsulatedPseudomonas flourescens cells in soil. Appl Microbiol Biotechnol 39:637–643
Vandenhove H, Merck R, Wilmots H, Vlassak K (1991) Survival ofPseudomonas fluorescens inocula of different physiological stages in soil. Soil Biol Biochem 23:1133–1142
Wessendorf J, Lingens F (1989) Effect of culture and soil conditions on survival ofPseudomonas fluorescens R1 in soil. Appl Microbiol Biotechnol 31:97–102
Yeates GW, Bamforth SS, Ross DJ, Tate KR, Sparling GP (1991) Recolonization of methyl bromide sterilized soils under four different field conditions. Biol Fertil Soil 11:181–189
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weir, S.C., Dupuis, S.P., Providenti, M.A. et al. Nutrient-enhanced survival of and phenanthrene mineralization by alginate-encapsulated and freePseudomonas sp. UG14Lr cells in creosote-contaminated soil slurries. Appl Microbiol Biotechnol 43, 946–951 (1995). https://doi.org/10.1007/BF02431932
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02431932