Abstract
The influence of buffered media upon the growth and alkaloid productivity of Catharanthus roseus hairy root culture was examined. As expected, the buffers minimized shifts in the pH of the media and had slightly negative effects upon growth. The growth of the hairy roots remained optimal in unbuffered media. The specific yield of lochnericine was significantly lower in response to the addition of buffers, while tabersonine was significantly higher. In contrast, the specific yields of ajmalicine, serpentine, and hörhammericine remained unchanged.
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Received: 5 May 1999 / Received revision: 8 September 1999 / Accepted: 24 September 1999
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Morgan, J., Barney, C., Penn, A. et al. Effects of buffered media upon growth and alkaloid production of Catharanthus roseus hairy roots. Appl Microbiol Biotechnol 53, 262–265 (2000). https://doi.org/10.1007/s002530050018
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DOI: https://doi.org/10.1007/s002530050018