Abstract
Oxalate-induced crystalline kidney injury is one of the most common types of crystalline nephropathy. Unfortunately, there is no effective treatment to reduce the deposition of calcium oxalate crystals and alleviate kidney damage. Thus, proactive therapeutic is urgently needed to alleviate the suffering it causes to patient. Here, we investigated whether IL-22 exerted nephroprotective effects to sodium oxalate-mediated kidney damage and its potential mechanism. Crystalline kidney injury models were developed in vitro and in vivo that was often observed in clinic. We provided evidence that IL-22 could effectively decrease the accumulation of ROS and mitochondrial damage in cell and animal models and reduce the death of TECs. Moreover, IL-22 decreased the expression of the NLRP3 inflammasome and mature IL-1β in renal tissue induced by sodium oxalate. Further studies confirmed that IL-22 could play an anti-inflammatory role by reducing the levels of cytokines such as IL-1β, IL-18, and TNF-α in serum. In conclusion, our study confirmed that IL-22 has protective effects on sodium oxalate-induced crystalline kidney injury by reducing the production of ROS, protecting mitochondrial membrane potential, and inhibiting the inflammatory response. Therefore, IL-22 may play a potential preventive role in sodium oxalate-induced acute renal injury.
Key points
• IL-22 could reduce sodium oxalate-mediated cytotoxicity and ameliorate renal injury.
• IL-22 could alleviate oxidative stress and mitochondrial dysfunction induced by sodium oxalate.
• IL-22 could inhibit inflammatory response of renal injury caused by sodium oxalate.
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This study was supported by National Natural Science Foundation of China (82073752, 81773620, 32070935, 31872746) and Shanghai Science and Technology Fund (20S11904700, 20JC1411000).
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HL, XM, and DJ designed the study and revised the paper. YG performed the major part of the research and drafted the manuscript. YS and WC carried out some confocal microscopy experiments. YM and HH performed the western blot assay. All authors approved the final version of the manuscript.
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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All animal experiments were conducted with the approval of the Ethics Committee of Fudan University School of Pharmacy (2019-06-YL-LHR-01).
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Gu, Y., Shen, Y., Chen, W. et al. Protective effects of interleukin-22 on oxalate-induced crystalline renal injury via alleviating mitochondrial damage and inflammatory response. Appl Microbiol Biotechnol 106, 2637–2649 (2022). https://doi.org/10.1007/s00253-022-11876-4
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DOI: https://doi.org/10.1007/s00253-022-11876-4