Abstract
The microbial community within the root system, the rhizosphere closely connected to the root, and their symbiotic relationship with the host are increasingly seen as possible drivers of natural pathogen resistance. Resistant cultivars have the most effective strategy in controlling the Chinese wheat yellow mosaic disease, but the roles of the root and rhizosphere microbial interactions among different taxonomic levels of resistant cultivars are still unknown. Thus, we aimed to investigate whether these microbial community composition and network characteristics are related to disease resistance and to analyze the belowground plant-associated microflora. Relatively high microbial diversity and stable community structure for the resistant cultivars were detected. Comparison analysis showed that some bacterial phyla were significantly enriched in the wheat root or rhizosphere of the resistant wheat cultivar. Furthermore, the root and rhizosphere of the resistant cultivars greatly recruited many known beneficial bacterial and fungal taxa. In contrast, the relative abundance of potential pathogens was higher for the susceptible cultivar than for the resistant cultivar. Network co-occurrence analysis revealed that a much more complex, more mutually beneficial, and a higher number of bacterial keystone taxa in belowground microbial networks were displayed in the resistant cultivar, which may have been responsible for maintaining the stability and ecological balance of the microbial community. Overall, compared with the susceptible cultivar, the resistant cultivar tends to recruit more potential beneficial microbial groups for plant and rhizosphere microbial community interactions. These findings indicate that beneficial rhizosphere microbiomes for cultivars should be targeted and evaluated using community compositional profiles.
Key points
• Different resistance levels in cultivars affect the rhizosphere microbiome..
• Resistant cultivars tend to recruit more potential beneficial microbial groups.
• Bacteria occupy a high proportion and core position in the microflora network.
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All data generated and/or analyzed during this study are included in this published article and its supplementary files.
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This work was supported by the China Agriculture Research System from the Ministry of Agriculture of the P.R. China (CARS-03) and sponsored by the K.C. Wong Magna Fund of Ningbo University, China.
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GTD, YJ and CJP conceived and designed the research. WCF and WFF conducted experiments. ZHQ and DYW contributed analytical tools. WCF and CGX analyzed the data. WCF wrote the manuscript. All authors read and approved the manuscript.
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Wu, C., Wang, F., Zhang, H. et al. Enrichment of beneficial rhizosphere microbes in Chinese wheat yellow mosaic virus-resistant cultivars. Appl Microbiol Biotechnol 105, 9371–9383 (2021). https://doi.org/10.1007/s00253-021-11666-4
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DOI: https://doi.org/10.1007/s00253-021-11666-4