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Effects of addition of elicitors on rimocidin biosynthesis in Streptomyces rimosus M527

  • Applied genetics and molecular biotechnology
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Abstract

The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, is highly effective against a broad range of fungal plant pathogens, but at low yields. Elicitation is an effective method of stimulating the yield of bioactive secondary metabolites. In this study, the biomass and filtrate of a culture broth of Escherichia coli JM109, Bacillus subtilis WB600, Saccharomyces cerevisiae, and Fusarium oxysporum f. sp. cucumerinum were employed as elicitors to promote rimocidin production in S. rimosus M527. Adding culture broth and biomass of S. cerevisiae (A3) and F. oxysporum f. sp. cucumerinum (B4) resulted in an increase of rimocidin production by 51.2% and 68.3% respectively compared with the production under normal conditions in 5-l fermentor. In addition, quantitative RT-PCR analysis revealed that the transcriptions of ten genes (rimA to rimK) located in the gene cluster involved in rimocidin biosynthesis in A3 or B4 elicitation experimental group were all higher than those of a control group. Using a β-glucuronidase (GUS) reporter system, GUS enzyme activity assay, and Western blot analysis, we discovered that elicitation of A3 or B4 increased protein synthesis in S. rimosus M527. These results demonstrate that the addition of elicitors is a useful approach to improve rimocidin production.

Key Points

• An effective strategy for enhancing rimocidin production in S. rimosus M527 is demonstrated.

• Overproduction of rimocidin is a result of higher expressed structural genes followed by an increase in protein synthesis.

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Contributors

ZQ Song conducted the experiments and wrote this article. Z Ma and A Bechthold designed the research and revised this article. XP Yu checked the final version. All authors read and approved the manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (31772213, 31972320), excellent youth fund of Zhejiang province, China (LR17C140002).

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou, 310018, Zhejiang Province, People’s Republic of China

    Zhangqing Song, Zheng Ma & Xiaoping Yu

  2. Institute for Pharmaceutical Sciences, Pharmaceutical Biology and Biotechnology, University of Freiburg, 79104, Freiburg, Germany

    Andreas Bechthold

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  1. Zhangqing Song
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  2. Zheng Ma
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  4. Xiaoping Yu
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Correspondence to Zheng Ma.

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Song, Z., Ma, Z., Bechthold, A. et al. Effects of addition of elicitors on rimocidin biosynthesis in Streptomyces rimosus M527. Appl Microbiol Biotechnol 104, 4445–4455 (2020). https://doi.org/10.1007/s00253-020-10565-4

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