Abstract
The effects of fungal elicitor on the physicochemical and microbial responses of Streptomyces natalensis HW-2 were investigated. The results showed that the elicitor could decrease dry cell weight (DCW) by 17.7% and increase the utilization of glucose, while the curve of pH was not obviously altered. The elicitor enhanced the yield of natamycin from 1.33 to 2.49 g/L. The morphology of the colony and the mycelium treated with elicitor showed significant differences from that of control. The level of intracellular reactive oxygen species (ROS) increased to 333.8 ng/L, which was a twofold increase comparing with the control. The concentration of Ca2+ reached 421.1 nmol/L, which increased by 32.8% after the addition of the elicitor. The activities of pyruvic carboxylase and phosphoenol pyruvate carboxylase were enhanced by 27.8 and 11.9%, respectively, while citrate synthase activity decreased by 23.1% in comparison with the control.
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Acknowledgements
We thank the National Natural Science Foundation of China (no. 31401672), Fund of Scientific and Technological Project in Henan Province (no.162102110056), and Fund of Young Teachers in Colleges and Universities of Henan Province (2016GGJS-060) for the financial support.
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Wang, D., Wei, L., Zhang, Y. et al. Physicochemical and microbial responses of Streptomyces natalensis HW-2 to fungal elicitor. Appl Microbiol Biotechnol 101, 6705–6712 (2017). https://doi.org/10.1007/s00253-017-8440-0
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DOI: https://doi.org/10.1007/s00253-017-8440-0