Abstract
The homologous transcriptional regulators ScbR and ScbR2 have previously been identified as γ-butyrolactone (GBL) and antibiotic receptors, respectively. They regulate diverse physiological processes in Streptomyces coelicolor in response to GBL and antibiotic signals. In this study, ScbR and ScbR2 proteins were shown to interact using a bacterial two-hybrid system where adenylate cyclase activity was reconstituted in Escherichia coli BTH101. These ScbR/ScbR2 interactions in S. coelicolor were then demonstrated by co-immunoprecipitation. The ScbR/ScbR2 heterodimer was shown to co-exist with their ScbR and ScbR2 respective homodimers. When potential operator targets in S. coelicolor were investigated, the heterodimer was found to bind in the promoter region of sco5158, which however was not a target for ScbR or ScbR2 homodimers. These results revealed a new mechanism of regulation by ScbR and ScbR2 in S. coelicolor.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant 31570031) and by the UK Biotechnology and Biological Sciences Research Council Grant BB/M022765/1. We thank Professor Mervyn Bibb (John Innes Centre) for providing plasmid pIJ10500 and Professor Emmanuelle Bouveret (Aix-Marseille University) for providing plasmids pKT25linker and pUT18Clinker and Dr. Keqiang Fan and Jinmin Gao for the help in figure processing.
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Li, X., Wang, J., Shi, M. et al. Evidence for the formation of ScbR/ScbR2 heterodimers and identification of one of the regulatory targets in Streptomyces coelicolor . Appl Microbiol Biotechnol 101, 5333–5340 (2017). https://doi.org/10.1007/s00253-017-8275-8
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DOI: https://doi.org/10.1007/s00253-017-8275-8