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Process development of itaconic acid production by a natural wild type strain of Aspergillus terreus to reach industrially relevant final titers

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Abstract

Itaconic acid is a promising organic acid and is commercially produced by submerged fermentation of Aspergillus terreus. The cultivation process of the sensitive filamentous fungus has been studied intensively since 1932, with respect to fermentation media components, oxygen supply, shearing rate, pH value, or culture method. Whereas increased final titers were achieved over the years, the productivity has so far remained quite low. In this study, the impact of the pH on the itaconic acid production was investigated in detail. The pH during the growth and production phase had a significant influence on the final itaconic acid concentration and pellet diameter. The highest itaconic acid concentration of 160 g/L was achieved at a 1.5-L scale within 6.7 days by raising and controlling the pH value to pH 3.4 in the production phase. An ammonia solution and an increased phosphate concentration were used with an itaconic acid yield of 0.46 (w/w) and an overall productivity of 0.99 g/L/h in a fed-batch mode. A cultivation with a lower phosphate concentration resulted in an equal final concentration with an increased yield of 0.58 (w/w) after 11.8 days and an overall productivity of 0.57 g/L/h. This optimized process was successfully transferred from a 1.5-L scale to a 15-L scale. After 9.7 days, comparable pellet morphology and a final concentration of 150 g/L itaconic acid was reached. This paper provides a process strategy to yield a final titer of itaconic acid from a wild-type strain of A. terreus which is in the same range as the well-known citric acid production.

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Acknowledgments

The authors thank Dr. Sylvia Kratz and Ms. Ursula Krüger of the Julius Kühn Institute for Crop and Soil Science (Braunschweig, Germany) for ICP-MS measurements.

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Authors and Affiliations

  1. Thünen Institute of Agricultural Technology, Bundesallee 50, 38116, Braunschweig, Germany

    Susan Krull, Antje Hevekerl, Anja Kuenz & Ulf Prüße

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  1. Susan Krull
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  2. Antje Hevekerl
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  3. Anja Kuenz
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  4. Ulf Prüße
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Correspondence to Anja Kuenz.

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Funding

This research is funded by the German Federal Ministry of Food and Agriculture, following a decision of the German Bundestag, via the Agency of Renewable Resources (Grant No. 22029312).

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Krull, S., Hevekerl, A., Kuenz, A. et al. Process development of itaconic acid production by a natural wild type strain of Aspergillus terreus to reach industrially relevant final titers. Appl Microbiol Biotechnol 101, 4063–4072 (2017). https://doi.org/10.1007/s00253-017-8192-x

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  • DOI: https://doi.org/10.1007/s00253-017-8192-x

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