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Lactobacillus plantarum HAC01 regulates gut microbiota and adipose tissue accumulation in a diet-induced obesity murine model

  • Applied microbial and cell physiology
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Abstract

The functional features of Lactobacillus plantarum HAC01 (HAC01), isolated from fermented Korean kimchi, were studied with regard to the fat mass, immunometabolic biomarkers and dysbiosis in a diet-induced obesity (DIO) murine model. L. rhamnosus GG (LGG) served as reference strain and a PBS-treated group as control. The administration of L. plantarum HAC01 resulted in reduction of the mesenteric adipose depot, the conjunctive tissue closely associated with the gastrointestinal tract, where lipid oxidative gene expression was upregulated compared to the control group. Metagenome analysis of intestinal microbiota showed that both strains HAC01 and LGG influenced specific bacterial families such as the Lachnospiraceae and Ruminococcaceae rather than the phyla Firmicutes and Bacteroidetes as a whole. The relative abundance of the Lachnospiraceae (phylum Firmicutes) was significantly higher in both LAB-treated groups than in the control. Comparing the impact of the two Lactobacillus strains on microbial composition in the gut also suggests strain-specific effects. The study emphasises the need for deeper studies into functional specificity of a probiotic organism at the strain level. Alleviation of obesity-associated dysbiosis by modulation of the gut microbiota appears to be associated with “indicator” bacterial taxa such as the family Lachnospiraceae. This may provide further insight into mechanisms basic to the mode of probiotic action against obesity and associated dysbiosis.

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Acknowledgements

This research was supported by the Korea Institute of Planning and Evaluation Technology in the Ministry of Food, Agriculture, Forestry and Fisheries (IPET), as part of the research project “Modulation of the microbiome with a concomitant anti-obesity effect by Kimchi originated probiotic feeding” (911053-1). We also gratefully acknowledge support from the Bio- and Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning (2016M3A9A5923160).

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Authors and Affiliations

  1. Advanced Green Energy and Environment Institute (AGEE), Handong Global University, 558 Handong-Ro, 3, Heunghae-eup, Buk-gu, Pohang, Gyungbuk, 37673, South Korea

    Soyoung Park, Yosep Ji, Hyunjoon Park & Wilhelm H. Holzapfel

  2. R&D Center, Pohang, Gyungbuk, 37668, South Korea

    Soyoung Park & Hoe-Yune Jung

  3. Department of Life Science, Division of Integrative Biosciences and Biotechnology, POSTECH, Pohang, Gyungbuk, 37673, South Korea

    Hoe-Yune Jung & Kyong-Tai Kim

  4. AtoGen Co. Ltd., 11-8 Techno 1-ro, Yuseong-gu, Daejeon, 34015, South Korea

    Jihee Kang & Sang-Haeng Choi

  5. School of Life Sciences, Handong Global University, Pohang, Gyungbuk, 791-708, South Korea

    Heuynkil Shin & Chang-Kee Hyun

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  1. Soyoung Park
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  2. Yosep Ji
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  9. Kyong-Tai Kim
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Correspondence to Wilhelm H. Holzapfel.

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All applicable international, national and institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

Ethical approval

All animal experiments and protocols were approved by the Committee on the Ethics of Animal Experiments of Handong Global University and were in agreement with the guidelines set forth by the Korean Association for Laboratory Animals.

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

WH was funded by Ministry of Food, Agriculture, Forestry and Fisheries (IPET), as part of the research project “Modulation of the microbiome with a concomitant anti-obesity effect by Kimchi originated probiotic feeding” (911053-1).

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Park, S., Ji, Y., Jung, HY. et al. Lactobacillus plantarum HAC01 regulates gut microbiota and adipose tissue accumulation in a diet-induced obesity murine model. Appl Microbiol Biotechnol 101, 1605–1614 (2017). https://doi.org/10.1007/s00253-016-7953-2

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