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The C-terminal MIR-containing region in the Pmt1 O-mannosyltransferase restrains sporulation and is dispensable for virulence in Beauveria bassiana

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Abstract

Protein O-mannosyltransferases (Pmts) belong to a highly conserved protein family responsible for the initiation of O-glycosylation of many proteins. Pmts contain one dolichyl-phosphate-mannose-protein mannosyltransferases (PMT) domain and three MIR motifs (mannosyltransferase, inositol triphosphate, and ryanodine receptor) that are essential for activity in yeast. We report that in the insect fungal pathogen, Beauveria bassiana, deletion of the C-terminal Pmt1 MIR-containing region (Pmt1∆ 311–902) does not alter O-mannosyltransferase activity, but does increase total cell wall protein O-mannosylation levels and results in phenotypic changes in fungal development and cell wall stability. B. bassiana mutants harboring the Pmt1 ∆ 311–902 mutation displayed a significant increase in conidiation with up-regulation of conidiation-associated genes and an increase in biomass accumulation as compared to the wild-type parent. However, decreased vegetative growth and blastospore production was noted, and Pmt1 ∆ 311–902 mutants were altered in cell wall composition and cell surface features. Insect bioassays revealed little effect on virulence for the Pmt1 ∆ 311–902 strain via cuticle infection or intrahemocoel injection assays, although differences in hyphal body differentiation in the host hemolymph and up-regulation of virulence-associated genes were noted. These data suggest novel roles for Pmt1 in negatively regulating conidiation and demonstrate that the C-terminal Pmt1 MIR-containing region is dispensable for enzymatic activity and organismal virulence.

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Acknowledgments

We thank Dr. Hai-Ying Wang (Institute of Nuclear-Agricultural Science, Zhejiang University) for technical assistance with PMT activity assay.

Funding

This study was funded by the Natural Science Foundation of China (grant numbers 31171902 and 31371993) and the Ministry of Science and Technology of China (grant number 2011AA10A204–4).

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Authors and Affiliations

  1. College of Plant Protection, Biotechnology Research Center, Southwest University, Chongqing, 400715, People’s Republic of China

    Zhangjiang He, Linli Luo, Xiaodong Yu & Yongjun Zhang

  2. Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida, 32611, USA

    Nemat O. Keyhani

  3. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, People’s Republic of China

    Shenghua Ying

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  1. Zhangjiang He
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He, Z., Luo, L., Keyhani, N.O. et al. The C-terminal MIR-containing region in the Pmt1 O-mannosyltransferase restrains sporulation and is dispensable for virulence in Beauveria bassiana . Appl Microbiol Biotechnol 101, 1143–1161 (2017). https://doi.org/10.1007/s00253-016-7894-9

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