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Investigation on antimicrobial agents of the terrestrial Streptomyces sp. BCC71188

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Abstract

The terrestrial actinomycete strain BCC71188 was identified as Streptomyces by its morphology (having spiral chain spore on the aerial mycelium), chemotaxonomy (containing LL-diaminopimelic acid in the cell wall), and 16S rRNA gene sequence analysis [showing high similarity values compared with Streptomyces samsunensis M1463T (99.85 %) and Streptomyces malaysiensis NBRC 16446T (99.40 %)]. The crude extract exhibited antimalarial against Plasmodium falciparum (IC50 0.19 μg/ml), anti-TB against Mycobacterial tuberculosis (MIC 6.25 μg/ml), and antibacterial against Bacillus cereus (MIC 1.56 μg/ml) activities. Therefore, chemical investigation was conducted by employing bioassay-guided method and led to the isolation of 19 compounds including two cyclic peptides (1–2), five macrolides (3–7), new naphthoquinone (8), nahuoic acid C (9), geldanamycin derivatives (10–13), cyclooctatin (14), germicidins A (15) and C (16), actinoramide A (17), abierixin, and 29-O-methylabierixin. These isolated compounds were evaluated for antimicrobial activity, such as antimalarial, anti-TB, and antibacterial activities, and for cytotoxicity against both cancerous (MCF-7, KB, NCI-H187) and non-cancerous (Vero) cells. Compounds 1–7, 10–14 exhibited antimalarial (IC50 0.22–7.14 μg/ml), and elaiophylin analogs (4–6) displayed anti-TB (MIC 0.78–12.00 μg/ml) and B. cereus (MIC 0.78–3.13 μg/ml) activities. Compounds 1, 2, 14, and abierixin displayed weak cytotoxicity, indicating a potential for antimicrobial agents.

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Acknowledgments

The National Center for Genetic Engineering and Biotechnology (BIOTEC) and Grant for International Research Integration: Research Pyramid, Rachadapiseksomphot Endowment Fund (GCURP_58_01_33_01), Chulalongkorn University are acknowledged for the financial support. K.S. also thanks Rachadapiseksomphot Endowment Fund from Chulalongkorn University for his postdoctoral fellowship.

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  1. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Khomsan Supong & Somboon Tanasupawat

  2. Department of Biotechnology, Faculty of Science, Burapha University, Chonburi, 20131, Thailand

    Paranee Sripreechasak

  3. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Paholyothin Road, Klong Luang, Pathumthani, 12120, Thailand

    Kannawat Danwisetkanjana, Pranee Rachtawee & Pattama Pittayakhajonwut

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Correspondence to Pattama Pittayakhajonwut.

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This study was funded by the National Center for Genetic Engineering and Biotechnology (BIOTEC) and Rachadapiseksomphot Endowment Fund from Chulalongkorn University (GCURP_58_01_33_01).

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Supong, K., Sripreechasak, P., Tanasupawat, S. et al. Investigation on antimicrobial agents of the terrestrial Streptomyces sp. BCC71188. Appl Microbiol Biotechnol 101, 533–543 (2017). https://doi.org/10.1007/s00253-016-7804-1

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