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Systematic optimization of fed-batch simultaneous saccharification and fermentation at high-solid loading based on enzymatic hydrolysis and dynamic metabolic modeling of Saccharomyces cerevisiae

  • Bioenergy and biofuels
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Abstract

An integrative simultaneous saccharification and fermentation (SSF) modeling is a useful guiding tool for rapid process optimization to meet the techno-economic requirement of industrial-scale lignocellulosic ethanol production. In this work, we have developed the SSF model composing of a metabolic network of a Saccharomyces cerevisiae cell associated with fermentation kinetics and enzyme hydrolysis model to quantitatively capture dynamic responses of yeast cell growth and fermentation during SSF. By using model-based design of feeding profiles for substrate and yeast cell in the fed-batch SSF process, an efficient ethanol production with high titer of up to 65 g/L and high yield of 85 % of theoretical yield was accomplished. The ethanol titer and productivity was increased by 47 and 41 %, correspondingly, in optimized fed-batch SSF as compared to batch process. The developed integrative SSF model is, therefore, considered as a promising approach for systematic design of economical and sustainable SSF bioprocessing of lignocellulose.

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Acknowledgments

This work has been financially supported by the Thailand Research Fund (Grant No. P-15-51025) and the National Center for Genetic Engineering and Biotechnology, Thailand (Grant No. P-15-50042). The authors would like to thank Miss Rujirek Nopgason and Mr. Nakul Rattanaphan for all the help during pretreatment and SSF experiments.

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Authors and Affiliations

  1. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani, 12120, Thailand

    Pornkamol Unrean, Sutamat Khajeeram & Kobkul Laoteng

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  1. Pornkamol Unrean
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  2. Sutamat Khajeeram
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  3. Kobkul Laoteng
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Correspondence to Pornkamol Unrean.

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Unrean, P., Khajeeram, S. & Laoteng, K. Systematic optimization of fed-batch simultaneous saccharification and fermentation at high-solid loading based on enzymatic hydrolysis and dynamic metabolic modeling of Saccharomyces cerevisiae . Appl Microbiol Biotechnol 100, 2459–2470 (2016). https://doi.org/10.1007/s00253-015-7173-1

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  • DOI: https://doi.org/10.1007/s00253-015-7173-1

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