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Temporal–spatial variation of bacterial diversity in estuary sediments in the south of Zhejiang Province, China

  • Environmental biotechnology
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Abstract

The winter and summer microbial community structure in sediment samples obtained from the estuaries of the wastewater-polluted River Ou (DO and XO), River Feiyun (DF and XF), and River Ao (DA and XA) in the south of Zhejiang Province in China was determined using 454 pyrosequencing. Sediment samples (DD and XD) were also correspondingly collected near the shore far from the estuaries for comparison. For the above sediments, 294,870 effective sequences were obtained to do the bacterial diversity and abundance determination. In total, 1924, 1517, 2071, 1956, 1995, 1800, 2261, and 2097 operational taxonomic units were obtained at 3 % distance cutoff in the DO, XO, DF, XF, DA, XA, DD, and XD sediments, respectively. Bacterial phylotype richness in DD was higher than the other sediments, and XO had the least richness. The most dominant class in the DA, DD, DF, DO, and XA sediments is Gammaproteobacteria. Deltaproteobacteria is the most dominant one in XD, XO, and XF. Circa 14.4 % sequences in XD were found to be affiliated with the Flavobacteriales order. Characterization of the estuarine sediment bacterial communities indicated that chemical pollution has the potential to decrease the natural variability that exists among estuary ecosystems. However, chemical pollutants did not cause clear bio-homogenization in these estuaries.

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Authors and Affiliations

  1. Institute for Eco-Environmental Sciences, Wenzhou Vocational College of Science & Technology, Wenzhou, 325006, People’s Republic of China

    Xiao-Ming Lu

  2. Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

    Chang Chen

  3. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, People’s Republic of China

    Tian-Ling Zheng

  4. College of Hydraulic and Environment Engineering, China Three Gorges University, Yichang, 443002, People’s Republic of China

    Jian-Jun Chen

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  1. Xiao-Ming Lu
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Correspondence to Xiao-Ming Lu.

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Funding

This study was funded by Open Project Program of the Key Laboratory of Tropical Marine Bio-resources and Ecology, SCSIO, CAS (No. LMB141001); MEL Visiting Fellowship of State Key Laboratory of Marine Environmental Science, Xiamen University (No. MELRS 1507); the Science and Technology Program of Wenzhou, China (No. S20140004); and the National Natural Science Foundation of China (No. 41401586).

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The authors declare that they have no competing interests.

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Lu, XM., Chen, C., Zheng, TL. et al. Temporal–spatial variation of bacterial diversity in estuary sediments in the south of Zhejiang Province, China. Appl Microbiol Biotechnol 100, 2817–2828 (2016). https://doi.org/10.1007/s00253-015-7103-2

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  • DOI: https://doi.org/10.1007/s00253-015-7103-2

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