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Characterization of a novel β-cypermethrin-degrading Aspergillus niger YAT strain and the biochemical degradation pathway of β-cypermethrin

  • Environmental biotechnology
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Abstract

Aspergillus niger YAT strain was obtained from Chinese brick tea (Collection number: CGMCC 10,568) and identified on the basis of morphological characteristics and internal transcribed spacer (ITS) sequence. The strain could degrade 54.83 % of β-cypermethrin (β-CY; 50 mg L−1) in 7 days and 100 % of 3-phenoxybenzoic acid (3-PBA; 100 mg L−1) in 22 h. The half-lives of β-CY and 3-PBA range from 3.573 to 11.748 days and from 5.635 to 12.160 h, respectively. The degradation of β-CY and 3-PBA was further described using first-order kinetic models. The pathway and mechanism of β-CY degraded by YAT were investigated by analyzing the degraded metabolites through high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Relevant enzymatic activities and substrate utilization were also investigated. β-CY degradation products were analyzed. Results indicated that YAT strain transformed β-CY into 3-PBA. 3-PBA was then gradually transformed into permethric acid, protocatechuic acid, 3-hydroxy-5-phenoxy benzoic acid, gallic acid, and phenol gradually. The YAT strain can also effectively degrade these metabolites. The results indicated that YAT strain has potential applications in bioremediation of pyrethroid insecticide (PI)-contaminated environments and fermented food.

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Acknowledgments

The authors extend their gratitude to the National Natural Science Foundation of China (31371775) for the financial support to this research.

Conflict of interest

We declare that no conflict of interest exists in the submission of this manuscript.

Ethical statement

All authors agreed to publish this manuscript. I would like to declare on behalf of my co-authors that this original work has not been previously published and is not under consideration for publication elsewhere, in whole or in part.

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Authors and Affiliations

  1. College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, 625014, People’s Republic of China

    Weiqin Deng, Derong Lin, Huaiyu Yuan, Zhilong Wang, Jianlong Li, Xinfeng Han, Kang Zhou, Li He, Xinjie Hu & Shuliang Liu

  2. College of Light Industry and Food, Sichuan University, Chengdu, Sichuan, 610065, People’s Republic of China

    Kai Yao

  3. The Laboratory of Microbiology, Dujiangyan Campus, Sichuan Agricultural University, Dujiangyan, Sichuan, 611830, People’s Republic of China

    Likou Zou

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  1. Weiqin Deng
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  2. Derong Lin
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  3. Kai Yao
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  4. Huaiyu Yuan
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  5. Zhilong Wang
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  9. Kang Zhou
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  10. Li He
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  11. Xinjie Hu
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Correspondence to Shuliang Liu.

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Weiqin Deng and Derong Lin contributed equally to this article.

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Deng, W., Lin, D., Yao, K. et al. Characterization of a novel β-cypermethrin-degrading Aspergillus niger YAT strain and the biochemical degradation pathway of β-cypermethrin. Appl Microbiol Biotechnol 99, 8187–8198 (2015). https://doi.org/10.1007/s00253-015-6690-2

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  • DOI: https://doi.org/10.1007/s00253-015-6690-2

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