Abstract
Pyrolysate from waste cotton was acid hydrolyzed and detoxified to yield pyrolytic sugars, which were fermented to ethanol by the strain Escherichia coli ACCC 11177. Mathematical models based on the fermentation data were also constructed. Pyrolysate containing an initial levoglucosan concentration of 146.34 g/L gave a glucose yield of 150 % after hydrolysis, suggesting that other compounds were hydrolyzed to glucose as well. Ethyl acetate-based extraction of bacterial growth inhibitors with an ethyl acetate/hydrolysate ratio of 1:0.5 enabled hydrolysate fermentation by E. coli ACCC 11177, without a standard absorption treatment. Batch processing in a fermenter exhibited a maximum ethanol yield and productivity of 0.41 g/g and 0.93 g/L·h−1, respectively. The cell growth rate (r x ) was consistent with a logistic equation \( {r}_x=0.21\left(1-\frac{X}{3.75}\right)X \), which was determined as a function of cell growth (X). Glucose consumption rate (r s ) and ethanol formation rate (r p ) were accurately validated by the equations \( {r}_s=0.25\frac{dX}{dt}+0.47X \) and \( {r}_p=0.05\frac{dX}{dt}+0.29X \), respectively. Together, our results suggest that combining mathematical models with fermenter fermentation processes can enable optimized ethanol production from cellulosic pyrolysate with E. coli. Similar approaches may facilitate the production of other commercially important organic substances.
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References
Balat M, Balat H (2009) Recent trends in global production and utilization of bio-ethanol fuel. Appl Energy 86:2273–2282
Bennett NM, Helle SS, Duff SJ (2009) Extraction and hydrolysis of levoglucosan from pyrolysate. Bioresour Technol 100(23):6059–6063
Chandel AK, Singh OV, da Silva SS (2011) Detoxification of lignocellulosic hydrolysates for improved bioethanol production. In: Dos Santos Bernardes MA (ed) Biofuel production-recent developments and prospects. ISBN: 978-953-307-478-8, In Tech pp 225–246
Chi Z, Rover M, Jun E, Deaton M, Johnston P, Brown RC, Wen Z, Jarboe LR (2013) Overliming detoxification of pyrolytic sugar syrup for direct fermentation of levoglucosan to ethanol. Bioresour Technol 150(C):220–227
Clark TA, Mackie KL (1984) Fermentation inhibitors in wood hydrolysates derived from the softwood Pinus radiata. J Chem Technol Biotechnol 34(B):101–110
Danon B, van der Aa L, de Jong W (2013) Furfural degradation in a dilute acidic and saline solution in the presence of glucose. Carbohydr Res 375:145–152
de Andrade RR, Rabelo SC, Filho FM, Filho RM, da Costa AC (2013) Evaluation of the alcoholic fermentation kinetics of enzymatic hydrolysates from sugarcane bagasse (Saccharum officinarum L.). J Chem Technol Biotechnol 88:1049–1057
Ding MZ, Wang X, Yang Y, Yuan YJ (2011) Metabolomic study of interactive effects of phenol, furfural, and acetic acid on Saccharomyces cerevisiae. OMICS 15:647–653
Du J, Liu P, Liu ZH, Sun DG, Tao CY (2010) Fast pyrolysis of biomass for bio-oil with ionic liquid and microwave irradiation. J Fuel Chem Technol 38(5):554–559
Garcia-Perez M, Wang SX, Shen J, Rhodes MJ, Tian FJ, Lee WJ, Wu H, Li CZ (2008a) Fast pyrolysis of oil mallee biomass: effect of temperature on the yield and quality of products. Ind Eng Chem Res 47:1846–1854
Garcia-Perez M, Wang SX, Shen J, Rhodes MJ, Lee WJ, Li CZ (2008b) Effects of temperature on the formation of lignin derived oligomers during the fast pyrolysis of mallee woody biomass. Energy Fuel 22:2022–2032
Jarboe LR, Chi Z (2013) Inhibition of microbial biocatalysts by biomass-derived aldehydes and methods for engineering tolerance. In: Luca T, Emilia P (eds) New developments in aldehydes research. Nova Science, Hauppauge, pp 101–120
Kawamoto H, Morisaki H, Saka S (2009) Secondary decomposition of levoglucosan in pyrolytic production from cellulosic biomass. J Anal Appl Pyrolysis 85(1–2):247–251
Klinke HB, Thomsen AB, Ahring BK (2004) Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass. Appl Microbiol Biotechnol 66(1):10–26
Layton DS, Ajjarapu A, Choi DW, Jarboe LR (2011) Engineering ethanologenic Escherichia coli for levoglucosan utilization. Bioresour Technol 102(17):8318–8322
Li L, Zhang HX (2004) Preparing levoglucosan derived from waste material by pyrolysis. Energy Sources 26(11):1053–1059
Lian J, Chen S, Zhou S, Wang Z, O’Fallon J, Li CZ, Garcia-Perez M (2010) Separation, hydrolysis and fermentation of pyrolytic sugars to produce ethanol and lipids. Bioresour Technol 101(24):9688–9699
Lian J, Garcia-Perez M, Chen S (2013) Fermentation of levoglucosan with oleaginous yeasts for lipid production. Bioresour Technol 133:183–191
Liu ZL, Moon J, Andersh BJ, Slininger PJ, Weber S (2008) Multiple gene-mediated NAD (P) H-dependent aldehyde reduction is a mechanism of in situ detoxification of furfural and 5-hydroxymethylfurfural by Saccharomyces cerevisiae. Appl Microbiol Biotechnol 81(4):743–753
Liu W, Lund H, Mathiesen BV, Zhang X (2011) Potential of renewable energy systems in China. Appl Energy 88(2):518–525
Lu Q, Xiong WM, Li WZ, Guo QX, Zhu XF (2009) Catalytic pyrolysis of cellulose with sulfated metal oxides: a promising method for obtaining high yield of light furan compounds. Bioresour Technol 100(20):4871–4876
Lu Q, Yang X, Dong C, Zhang Z, Zhang X, Zhu X (2011) Influence of pyrolysis temperature and time on the cellulose fast pyrolysis products: analytical Py-GC/MS study. J Anal Appl Pyrolysis 92(2):430–438
Luedeking R, Piret EL (1959) A kinetic study of the lactic acid fermentation. Batch process at controlled pH. J Biochem Microbiol Technol Eng 1(4):393–412
Lv D, Xu M, Liu X, Zhan Z, Li Z, Yao H (2010) Effect of cellulose, lignin, alkali and alkaline earth metallic species on biomass pyrolysis and gasification. Fuel Process Technol 91(8):903–909
Martinez A, Rodriguez ME, York SW, Preston JF, Ingram LO (2000) Effects of Ca(OH)2 treatments (“overliming”) on the composition and toxicity of bagasse hemicellulose hydrolysates. Biotechnol Bioeng 69(5):526–536
Nakagawa M, Sakai Y, Yasui T (1984) Itaconic acid fermentation of levoglucosan. J Ferment Technol 62:201–203
Oasmaa A, Czernik S (1999) Fuel oil quality of biomass pyrolysis oils—state of the art for the end users. Energy Fuel 13(4):914–921
Palmqvist E, Hahn-Hagerdal B (2000) Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresour Technol 74:25–33
Patil SKR, Lund CRF (2011) Formation and growth of humins via aldol addition and condensation during acid-catalyzed conversion of 5-hydroxymethylfurfural. Energy Fuel 25(10):4745–4755
Patwardhan PR, Satrio JA, Brown RC, Shanks BH (2010) Influence of inorganic salts on the primary pyrolysis products of cellulose. Bioresour Technol 101(12):4646–4655
Piskorz J, Majerski P, Radlein D, Vladars-Usas A, Scott DS (2000) Flash pyrolysis of cellulose for production of anhydro-oligomers. J Anal Appl Pyrolysis 56:145–166
Rinaldi R, Schüth F (2009) Acid hydrolysis of cellulose as the entry point into biorefinery schemes. ChemSusChem 2(12):1096–1107
Rover MR, Johnston PA, Jin T, Smith RG, Brown RC, Jarboe L (2014) Production of clean pyrolytic sugars for fermentation. ChemSusChem 7(6):1662–1668
Sharma RK, Wooten JB, Baliga VL, Lin X, Chan WG, Hajaligol MR (2004) Characterization of chars from pyrolysis of lignin. Fuel 83:1469–1482
Shimada N, Kawamoto H, Saka S (2008) Different action of alkali/alkaline earth metal chlorides on cellulose pyrolysis. J Anal Appl Pyrolysis 81(1):80–87
Sukhbaatar B, Li Q, Wan C, Yu F, Hassan EB, Steele P (2014) Inhibitors removal from bio-oil aqueous fraction for increased ethanol production. Bioresour Technol 161:379–384
Tsuchiya Y, Sumi K (1970) Thermal decomposition products of cellulose. J Appl Polym Sci 14(8):2003–2013
Wang P, Zhan S, Yu H, Xue X, Hong N (2010) The effects of temperature and catalysts on the pyrolysis of industrial wastes (herb residue). Bioresour Technol 101(9):3236–3241
Wu S, Tsui R (2012) Research and analysis on energy hot topics in China. R&D, Arup East Asia. http://www.driversofchange.com/projects/energy-hot-topics-in-china/
Yang Z, Zhang B, Chen X, Bai Z, Zhang H (2008) Studies on pyrolysis of wheat straw residues from ethanol production by solid-state fermentation. J Anal Appl Pyrolysis 81(2):243–246
Yu ZS, Zhang HX (2003a) Ethanol fermentation of acid-hydrolyzed cellulosic pyrolysate with Saccharomyces cerevisiae. Bioresour Technol 90(1):95–100
Yu ZS, Zhang HX (2003b) Preteatments of cellulose pyrolysate for ethanol production by Saccharomyces cerevisiae, Pichia sp. YZ-1 and Zymomonas mobilis. Biomass Bioenergy 24(3):257–262
Zhang XL, Li J, Yang WH, Blasiak W (2011) Formation mechanism of levoglucosan and formaldehyde during cellulose pyrolysis. Energy Fuel 25(8):3739–3746
Zhao X, Peng F, Du W, Liu C, Liu D (2012) Effects of some inhibitors on the growth and lipid accumulation of oleaginous yeast Rhodosporidium toruloides and preparation of biodiesel by enzymatic transesterification of the lipid. Bioprocess Biosyst Eng 35:993–1004
Zhuang XL, Zhang HX, Yang JZ, Qi HY (2001) Preparation of levoglucosan by pyrolysis of cellulose and its citric acid fermentation. Bioresour Technol 79(1):63–66
Acknowledgments
This work was supported by funding from the National Natural Science Foundation of China (Grants No. 21177153) and the Strategic Priority Research Program (B) of the Chinese Academy of Sciences (XDB15010200). We express our thanks to Dr. Zhihui Bai and Dr. Zhiguang Yang at the Research Center for Eco-Environmental Science in the Chinese Academy of Sciences.
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Chang, D., Yu, Z., Islam, Z.U. et al. Mathematical modeling of the fermentation of acid-hydrolyzed pyrolytic sugars to ethanol by the engineered strain Escherichia coli ACCC 11177. Appl Microbiol Biotechnol 99, 4093–4105 (2015). https://doi.org/10.1007/s00253-015-6475-7
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DOI: https://doi.org/10.1007/s00253-015-6475-7