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Comparison of the biological activities of Saccharomyces cerevisiae-expressed intracellular EGF, extracellular EGF, and tagged EGF in early-weaned pigs

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Abstract

Epidermal growth factor (EGF) ameliorates stress and prevents incomplete gastrointestinal development in early-weaned piglets in commercial swine farming. This study aimed to further analyze the biological activities of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-EGF) from Saccharomyces cerevisiae in early-weaned pigs. In this study, we assigned 24 pigs to each of 5 groups that were provided a basic diet (the control group) or a diet supplemented with empty vector-expressing S. cerevisiae [the INVSc1(EV) group], T-EGF-expressing S. cerevisiae [the INVSc1-TE(−) group], EE-EGF-expressing S. cerevisiae [the INVSc1-EE(+) group], or IE-EGF-expressing S. cerevisiae [the INVSc1-IE(+) group]. All treatments were delivered at a dose of 60 μg EGF/kg body weight (BW) everyday. All the piglets were sacrificed after 21 day to determine their physio-biochemical indexes, immune functions, and intestinal development. In the piglet experiments, recombinant S. cerevisiae survived throughout the intestinal tract. The BW and intestinal development (e.g., mean villous height, crypt depth, villous height:crypt depth ratio (IVR), and total protein, DNA, and RNA contents) of the piglets were significantly enhanced in the INVSc1-IE(+) group compared with the animals in the INVSc1-EE(+) and INVSc1-TE(−) groups (P < 0.05). In addition, increased proliferating cell nuclear antigen (PCNA) staining was observed in the piglets that received the INVSc1-IE(+) treatment (approximately 80 %) compared with those that received the INVSc1-TE(−) (approximately 70 %) and INVSc1-EE(+) treatments (approximately 70 %). The levels of lactate dehydrogenase (LDH), creatine kinase (CK), alkaline phosphatase (ALP), immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were also significantly increased in the INVSc1-IE(+) group compared with the INVSc1-EE(+) and INVSc1-TE(−) groups (P < 0.05). Furthermore, the proliferation of piglet enterocytes was also significantly stimulated by both IE-EGF and EE-EGF compared with T-EGF in vitro (P < 0.05). Our data further demonstrate the previously reported hypothesis that IE-EGF is more suitable than EE-EGF or T-EGF for applications in early-weaned pigs.

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Acknowledgments

We thank the teachers and workers at Shenzhen Premix Nutrition CO., LTD. This study was financially supported by the Ministry of Science and Technology of Sichuan Province (2012NZ0033) and Animal Science Discipline Program of Southwest University for Nationalities (2014XWD–S0905).

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    Authors and Affiliations

    1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, People’s Republic of China

      Shujin Wang, Chunhua Guo, Zhengfan Zhang, Yanling Huang & Xue Bai

    2. Shenzhen Premixinve Nutrition Co., LTD, Shenzhen, 518103, People’s Republic of China

      Lin Zhou & Kuanmin Yang

    3. Key Laboratory of Animal Genetics and Breeding, State Ethnic Affairs Commission and Ministry of Education, Chengdu, 610041, People’s Republic of China

      Shujin Wang

    4. Animal Science Research Institute of Sichuan Province, Chengdu, 610041, People’s Republic of China

      Jiabao Yang

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    1. Shujin Wang
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    Correspondence to Chunhua Guo or Lin Zhou.

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    Chunhua Guo and Lin Zhou contributed equally to this work.

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    Wang, S., Guo, C., Zhou, L. et al. Comparison of the biological activities of Saccharomyces cerevisiae-expressed intracellular EGF, extracellular EGF, and tagged EGF in early-weaned pigs. Appl Microbiol Biotechnol 99, 7125–7135 (2015). https://doi.org/10.1007/s00253-015-6468-6

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