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Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10

  • Applied genetics and molecular biotechnology
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Abstract

The roles of many sigma factors are unclear in regulatory mechanism of secondary metabolism in Streptomyces. Here, we report the regulation network of a group 3 sigma factor, WhiGch, from a natamycin industrial strain Streptomyces chattanoogensis L10. WhiGch regulates the growth and morphological differentiation of S. chattanoogensis L10. The whiG ch deletion mutant decreased natamycin production by about 30 % and delayed natamycin production more than 24 h by delaying the growth. Overexpression of the whiG ch gene increased natamycin production in large scale production medium by about 26 %. WhiGch upregulated the transcription of natamycin biosynthetic gene cluster and inhibited the expression of migrastatin and jadomycin analog biosynthetic polyketide synthase genes. WhiGch positively regulated natamycin biosynthetic gene cluster by directly binding to the promoters of scnC and scnD, which were involved in natamycin biosynthesis, and these binding sites adjacent to translation start codon were determined. Thus, this paper further elucidates the high natamycin yield mechanisms of industrial strains and demonstrates that a valuable improvement in the yield of the target metabolites can be achieved through manipulating the transcription regulators.

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Acknowledgments

This work was supported by Key Program of Zhejiang Provincial Natural Science Foundation of China (LZ12C01001), National Basic Research Program of China (973 Program) (No. 2012CB721005), and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20120101110143). We specially thank Dr. Gerald Zvobgo for reading this paper. We sincerely thank Chris Wood for English editing and proofreading. We are grateful to Dr. Yi-Ling Du for the critical advice on the work.

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China

    Shui-Ping Liu, Pin Yu, Peng-Hui Yuan, Zhen-Xing Zhou, Qing-Ting Bu, Xu-Ming Mao & Yong-Quan Li

  2. Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, Zhejiang, China

    Xu-Ming Mao & Yong-Quan Li

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  1. Shui-Ping Liu
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Correspondence to Yong-Quan Li.

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Liu, SP., Yu, P., Yuan, PH. et al. Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10. Appl Microbiol Biotechnol 99, 2715–2726 (2015). https://doi.org/10.1007/s00253-014-6307-1

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