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New insights into the structure and dynamics of actinomycetal community during manure composting

  • Environmental biotechnology
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Abstract

Despite advancing knowledge about the functional role of actinomycetes in degrading lignocellulosic materials, definitive knowledge concerning the diversity and dynamics of the actinomycetal community in composting is still lacking. In this study, real-time polymerase chain reaction (PCR) coupled with denaturing gradient gel electrophoresis (DGGE) and clone library construction were applied to investigate actinomycetal diversity and dynamics in a pilot-scale composting. Quantitative real-time PCR data revealed that actinomycetes accounted for 18–86 % of bacteria and that the fraction peaked during the maturing phase, indicating that Actinobacteria were critical to the compost ecosystem. Qualitatively, actinomycetal communities displayed distinct temporal variations during composting. Fourteen distinct genera of actinomycetes and an unknown group were observed in manure composts. Redundancy analysis indicated that temperature exerted an influence over the actinomycetal communities. Specifically, pathogenic Corynebacterium species dominated in the initial phase, whereas the genera Saccharomonospora and Thermobifida were abundant in the thermophilic phase. In maturing composts, mesophilic Micrococcineae members were most prevalent. The dominant thermophiles along with Micrococcineae may jointly facilitate the degradation of lignocellulosic materials during composting. Together, our research revealed a more detailed ecological and potential functional role for actinomycetes in the compost ecology.

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Acknowledgments

The authors thank P.J. Strong for his assistance in improving the manuscript. This work was financially supported by China National Critical Project for Science and Technology on Water Pollution Prevention and Control with Grant No. 2012ZX07101012.

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  1. Institute of Environmental Science and Technology, Zhejiang University, Yuhangtang Road 866#, Hangzhou, 310058, China

    Cheng Wang, Xiaohui Guo, Hui Deng, Da Dong, Qiaoping Tu & Weixiang Wu

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  1. Cheng Wang
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  2. Xiaohui Guo
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  3. Hui Deng
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  4. Da Dong
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  5. Qiaoping Tu
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Correspondence to Weixiang Wu.

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Wang, C., Guo, X., Deng, H. et al. New insights into the structure and dynamics of actinomycetal community during manure composting. Appl Microbiol Biotechnol 98, 3327–3337 (2014). https://doi.org/10.1007/s00253-013-5424-6

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  • DOI: https://doi.org/10.1007/s00253-013-5424-6

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