Abstract
Among several fatty acids tested, oleic acid was selected as the most efficient inducer for the production of 4-hydroxydodecanoic acid, a metabolite of β-oxidation, by Waltomyces lipofer. Cells were induced by incubation for 12 h in a medium containing 10 g l−1 yeast extract, 10 g l−1 peptone, 5 g l−1 oleic acid, 1 g l−1 glucose, and 0.05 % (w/v) Tween 80. The optimal reaction conditions for the production of γ-lactones by induced cells were pH 6.5, 35 °C, 200 rpm, 0.71 M Tris, 60 g l−1 hydroxy fatty acid, and 20 g l−1 cells. Non-induced cells produced 38 g l−1 γ-dodecalactone from 60 g l−1 10-hydroxystearic acid after 30 h, with a conversion yield of 63 % (w/w) and a productivity of 1.3 g l−1 h−1 under the optimized conditions, whereas induced cells produced 51 g l−1 γ-dodecalactone from 60 g l−1 10-hydroxystearic acid after 30 h, with a conversion yield of 85 % (w/w) and a productivity of 1.7 g l−1 h−1. The conversion yield and productivity of induced cells were 22 % and 1.3-fold higher, respectively, than those of non-induced cells. Induced cells also produced 28 g l−1 γ-decalactone and 12 g l−1 γ-butyrolactone from 60 g l−1 12-hydroxystearic acid and 60 g l−1 10-hydroxydecanoic acid, respectively, after 30 h. The concentration, conversion yield, and productivity of γ-dodecalactone and γ-decalactone are the highest reported thus far. This is the first study on the biotechnological production of γ-butyrolactone.
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This study was supported by a grant (no. 112002–3) from the Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.
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An, JU., Oh, DK. Increased production of γ-lactones from hydroxy fatty acids by whole Waltomyces lipofer cells induced with oleic acid. Appl Microbiol Biotechnol 97, 8265–8272 (2013). https://doi.org/10.1007/s00253-013-5089-1
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DOI: https://doi.org/10.1007/s00253-013-5089-1